ArticleOptica2025
Wide-field bond-selective fluorescence imaging: from single-molecule to cellular imaging beyond video rate.
Article in Optica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- The Next Frontier in Vibrational Imaging: Chemically Designed Raman Probes Pass the Spectral Barrier.JACS Au · 2026Review
- Far-field single-molecule vibrational spectroscopy and imaging.Chemical science · 2026Review
- Nonlinear optical microscopy with an obscuration-free, freeform reflective objective.Biomedical optics express · 2025Article
- Single-Molecule Vibrational Thermometry.The journal of physical chemistry. B · 2025Article
- Two-dimensional bond-selective fluorescence spectroscopy: violations of the resonance condition, vibrational cooling rate dispersion, and super-multiplex imaging.Chemical science · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Wide-field (WF) imaging is pivotal for observing dynamic biological events. While WF chemical microscopy offers high molecular specificity, it lacks the sensitivity for single-molecule detection. In contrast, WF fluorescence microscopy provides live-cell dynamic mapping but fails to leverage the rich chemical information necessary for functional interpretations. To address these limitations, we introduce Wide-Field Bond-selective Fluorescence-detected Infrared-Excited (WF-BonFIRE) spectro-microscopy. This technique combines rationally optimized imaging speed and field-of-view (FOV) to achieve single-molecule sensitivity with bond-selective contrast. We demonstrate WF-BonFIRE's capabilities in imaging single molecules, cells, astrocytes, and live neurons, capturing single FOVs up to 50 μm × 50 μm, with further expansion via multi-FOV mosaicking. Additionally, we have implemented a new temporal-delay modulation scheme that allows real-time kilohertz WF-BonFIRE imaging with speeds up to 1500 Hz. We showcase the millisecond temporal resolution through monitoring the random motion of live Escherichia coli. Leveraging its ability to distinguish molecules through distinct narrow-band BonFIRE signals, we further demonstrate multicolor real-time
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.