ArticleAmerican journal of translational research2025
miR-124 inhibits the proliferation, migration and invasion of esophageal squamous cell carcinoma by downregulating STATS.
Article in American journal of translational research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
objectiveTo investigate the expression, role and the underlying mechanism of miR-124 in esophageal squamous cell carcinoma (ESCC).
methodsA total of 25 pairs of ESCC and adjacent tissues were collected for analysis. The human normal esophageal epithelial cell line (Het-1A) and human esophageal cancer cell lines (EC-1) was cultured in Dulbecco's Modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS). Cells were transfected with miR-124 mimics and inhibitors. A series of assays, including Cell-Counting-Kit-8 (CCK-8), Transwell migration and invasion assays, real-time quantitative polymerase chain reaction (RT-qPCR), enzyme-linked immunosorbent assay (ELISA) and luciferase reporter gene assay, were performed to detect the effects of miR-124 on ESCC cells. Spearman's correlation analysis was used to evaluate the relationship of miR-124 expression and signal transducer and activator of transcription-3 (STAT3) expression.
resultsmiR-124 was significantly downregulated in ESCC tissues and cells. Overexpression of miR-124 inhibited the proliferation, migration and invasion of ESCC cells in vitro, while inhibition of miR-124 promoted these processes (all P<0.05). miR-124 was found to specifically bind to the 3'-untranslated region (3'UTR) of STAT3. The expression level of STAT3 was obviously higher in tumor tissues and cells compared to normal adjacent tissues and cells (all P<0.05). Moreover, miR-124 expression was negatively associated with STAT3 levels. Restoring STAT3 expression in ESCC cells transfected with miR-124 mimics partially reversed the inhibitory effects of miR-124 mimics on cell proliferation, migration, and invasion. Conversely, inhibiting STAT3 expression in ESCC cells transfected with miR-124 inhibitors partially abolished the promoting effects of miR-124 inhibitors on the proliferation, migration and invasion of ESCC cells.
conclusionmiR-124 inhibits the proliferation, migration and invasion of ESCC cells by downregulating STATS, indicating that miR-124 may serve as a molecular candidate for treating ESCC.
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