Evidence map›Paper›PMID 40950159›Full record

ArticlebioRxiv : the preprint server for biology2025

Cysteine reactivity profiling identifies host regulators of

John Neff, Kristen E DeMeester, Paola K Parraga, Radu Suciu, Melissa Dix, Gabriel Simon, Max A Gianakopoulos, Bruno Melillo, Benjamin F Cravatt, Michael U Shiloh

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

John NeffDepartment of Internal Medicine University of Texas Southwestern Medical Center Dallas, TX 75390, USA.
Kristen E DeMeesterDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Paola K ParragaDepartment of Internal Medicine University of Texas Southwestern Medical Center Dallas, TX 75390, USA.ORCID 0000-0003-0710-5414
Radu SuciuDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Melissa DixDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.
Gabriel SimonVividion Therapeutics, San Diego, California 92121, USA.
Max A GianakopoulosLafayette College, Easton, Pennsylvania 18042, USA.
Bruno MelilloDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.ORCID 0000-0002-9708-5287
Benjamin F CravattDepartment of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA.ORCID 0000-0001-5330-3492
Michael U ShilohDepartment of Internal Medicine University of Texas Southwestern Medical Center Dallas, TX 75390, USA.ORCID 0000-0003-4329-2253

Funding

RP5: Chemical proteomic discovery of small-molecule probes for autophagy proteinsU19AI142784 · NIAID · UT SOUTHWESTERN MEDICAL CENTER · PI VIRGIN, HERBERT W · 2019 to 2023
$36.9M
MOLECULAR MICROBIOLOGY TRAINING GRANTT32AI007520 · NIAID · UT SOUTHWESTERN MEDICAL CENTER · PI Julie K Pfeiffer · 1997 to 2026
$7.0M
Human airway microfold cells in mucosal immunity to bacterial pathogensU01AI125939 · NIAID · UT SOUTHWESTERN MEDICAL CENTER · PI SHILOH, MICHAEL · 2016 to 2020
$2.2M
NIAID NIH HHS T32 AI007520NIAID NIH HHS U01 AI125939NIAID NIH HHS U19 AI142784
6 · The paper itself

Abstract

Innate immune cells such as monocytes and macrophages provide the earliest defense against infection by intracellular pathogens by initiating signaling pathways and restricting pathogen replication. However, the full complement of proteins that mediate cell-autonomous immunity remains incompletely defined. Here, we applied cysteine-directed activity-based protein profiling (ABPP) to map proteome-wide cysteine reactivity changes in THP-1 monocytes and primary human monocyte-derived macrophages during

Indexed as

chemical proteomicscovalent probescysteineinnate immunitymacrophagemass spectrometryMycobacterium tuberculosis

Identifiers

PMID40950159
PMCPMC12424734

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.