Evidence map›Paper›PMID 40950106›Full record

ArticlebioRxiv : the preprint server for biology2025

Slice-PASEF: Maximising Ion Utilisation in LC-MS Proteomics.

Ludwig R Sinn, Lukasz Szyrwiel, Justus Grossmann, Kate Lau, Katharina Faisst, Di Qin, Florian Mutschler, Luke Khoury, Andrew Leduc, Markus Ralser and 6 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Ludwig R SinnDepartment of Biochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.ORCID 0000-0003-4692-0681
Lukasz SzyrwielDepartment of Biochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.ORCID 0000-0003-4692-0681
Justus GrossmannDepartment of Biochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Kate LauDepartment of Biochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Katharina FaisstDepartment of Biochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.
Di QinSpatial Proteomics Group, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.
Florian MutschlerProteome Dynamics, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.
Luke KhouryDepartments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center and Barnett Institute, Northeastern University, Boston, MA, USA; Parallel Squared Technology Institute, Watertown, MA, USA.ORCID 0000-0001-7794-6812
Andrew LeducDepartments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center and Barnett Institute, Northeastern University, Boston, MA, USA; Parallel Squared Technology Institute, Watertown, MA, USA.ORCID 0000-0001-6234-0623
Markus RalserDepartment of Biochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.ORCID 0000-0001-9535-7413
Fabian CosciaSpatial Proteomics Group, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.ORCID 0000-0002-2244-5081
Matthias SelbachProteome Dynamics, Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany.ORCID 0000-0003-2454-8751
Nikolai SlavovDepartments of Bioengineering, Biology, Chemistry and Chemical Biology, Single Cell Proteomics Center and Barnett Institute, Northeastern University, Boston, MA, USA; Parallel Squared Technology Institute, Watertown, MA, USA.ORCID 0000-0003-2035-1820
Nagarjuna NagarajEvotec International GmbH, Neuried, Germany.
Martin StegerNEOsphere Biotechnologies GmbH, Planegg, Germany.ORCID 0000-0003-1637-8190
Vadim DemichevDepartment of Biochemistry, Charité - Universitätsmedizin Berlin, Berlin, Germany.ORCID 0000-0002-2424-9412

Funding

Comprehensive and Cell Type-Specific Proteogenomic Profiling of Aberrant and Mis-Processed Proteins in Alzheimer's Disease and Tauopathy ModelsR01AG092460 · NIA · NORTHEASTERN UNIVERSITY · PI Mahlon Collins, BRADLEY T. HYMAN · 2025 to 2026
$1.5M
Molecular and metabolic influences on the activation of monocytes and macrophages at single-cell resolutionR35GM148218 · NIGMS · NORTHEASTERN UNIVERSITY · PI Nikolai Slavov · 2023 to 2026
$1.5M
NIA NIH HHS R01 AG092460NIGMS NIH HHS R35 GM148218
6 · The paper itself

Abstract

Quantitative mass spectrometry (MS)-based proteomics has become a streamlined technology with a wide range of usage. Many emerging applications, such as single-cell proteomics, spatial proteomics of tissue sections and the profiling of low-abundant posttranslational modifications, require the analysis of minimal sample amounts and are thus constrained by the sensitivity of the workflow. Here, we present Slice-PASEF, a mass spectrometry technology that leverages trapped ion mobility separation of ions to attain the theoretical maximum of tandem MS sensitivity. We implement Slice-PASEF using a new module in our DIA-NN software and show that Slice-PASEF uniquely enables precise quantitative proteomics of low sample amounts. We further demonstrate its utility towards a range of applications, including single cell proteomics and degrader drug screens via ubiquitinomics.

Identifiers

PMID40950106
PMCPMC12424990

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.