ArticlebioRxiv : the preprint server for biology2025
Human-mouse cross-species comparison identifies common and unique aspects of intestinal mesenchyme development.
Kelli Johnson, Xiangning Dong, Zhiwei Xiao, Hamza Islam, Meghan Anderman, Ian Glass, Jason R Spence, Katherine D Walton
Abstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
8 authors.
Kelli JohnsonDepartment of Internal Medicine, Division of Gastroenterology and Hepatology, University of Michigan Medical School, Ann Arbor, MI, USA.
Xiangning DongDepartment of Internal Medicine, Division of Gastroenterology and Hepatology, University of Michigan Medical School, Ann Arbor, MI, USA.
Zhiwei XiaoDepartment of Internal Medicine, Division of Gastroenterology and Hepatology, University of Michigan Medical School, Ann Arbor, MI, USA.
Hamza IslamDepartment of Internal Medicine, Division of Gastroenterology and Hepatology, University of Michigan Medical School, Ann Arbor, MI, USA.
Meghan AndermanDepartment of Internal Medicine, Division of Gastroenterology and Hepatology, University of Michigan Medical School, Ann Arbor, MI, USA.
Ian GlassDepartment of Pediatrics, Genetic Medicine, University of Washington, Seattle, WA 98195, USA.
Jason R SpenceDepartment of Internal Medicine, Division of Gastroenterology and Hepatology, University of Michigan Medical School, Ann Arbor, MI, USA.ORCID 0000-0001-7869-3992 Katherine D WaltonDepartment of Internal Medicine, Division of Gastroenterology and Hepatology, University of Michigan Medical School, Ann Arbor, MI, USA.ORCID 0000-0001-9108-5617 Funding
LABORATORY OF DEVELOPMENTAL BIOLOGYR24HD000836 · NICHD · UNIVERSITY OF WASHINGTON · PI Ian Amos Glass · 1995 to 2026
$16.9MConsortium on Intestinal Regeneration and Fetal Reversion: from Atlas to TherapyRC2DK140862 · NIDDK · CEDARS-SINAI MEDICAL CENTER · PI SARAH E BLUTT, DARIO BOFFELLI · 2024 to 2026
$6.0MNiche support of human intestinal stem cellsU01DK103141 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI SPENCE, JASON · 2014 to 2023
$3.9MMechanisms of Hedgehog Regulation of Intestinal Villus MorphogenesisR01DK121166 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI WALTON, KATHERINE DEMPSEY · 2019 to 2023
$1.8MHuman iPSC intestine mimetics with integrated mesenchymal, endothelial and enteric nervous tissueR01DK137806 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Jason Spence · 2024 to 2026
$1.5MInterrogating sub-epithelial cell heterogeneity in the developing human intestinal stem cell nicheF32DK138694 · NIDDK · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Kelli Johnson · 2024 to 2026
$234kNICHD NIH HHS R24 HD000836NIDDK NIH HHS F32 DK138694NIDDK NIH HHS R01 DK121166NIDDK NIH HHS R01 DK137806NIDDK NIH HHS RC2 DK140862NIDDK NIH HHS U01 DK103141
6 · The paper itselfAbstract
Using single-cell RNA sequencing (scRNA-seq) and histological approaches, we examine cross-species cellular identity, diversity and organization of mesenchymal (fibroblast) populations in the developing mouse and human intestines. In both species, we defined 7 fibroblast populations. Using cross-species integration and label transfer approaches we find that each mesenchymal cell subtype in the murine intestine is highly concordant to a cell type/state in the human intestine, and vice-versa, suggesting a strong conservation of mesenchymal cell types/states across species. Despite this conservation, we also observe that individual lineage-defining genes are not always shared and can be found in different mesenchymal populations. High resolution spatial analysis via fluorescent in situ hybridization (FISH) and immunofluorescence (IF) confirmed these findings and revealed that transcriptionally-defined sub-types of intestinal mesenchymal cells in mice and humans are organized within similar spatial domains.
Indexed as
cross-species comparisondevelopmentfibroblastmesenchymeregionalizationsingle-cell sequencingSmall intestinesmooth muscletranscript localizationvillus morphogenesis
Identifiers
PMID40950081
PMCPMC12424763
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LicenceCC BY-NC-ND
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