Lysyl oxidase-dependent bone marrow stiffening promotes neutrophil activation in diabetes.

Mahesh Agarwal, Sathishkumar Chandrakumar, Vihar Trada, Samanvaya Srivastava, Kaustabh Ghosh

Abstract readPreprint

In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mahesh AgarwalDepartment of Ophthalmology, University of California, Los Angeles, CA, USA 90095.ORCID 0000-0002-0529-0196

Sathishkumar ChandrakumarDoheny Eye Institute, Pasadena, CA, USA 91103.ORCID 0000-0003-2320-050X

Vihar TradaChemical and Biomolecular Engineering, University of California, Los Angeles, CA, USA 90095.

Samanvaya SrivastavaChemical and Biomolecular Engineering, University of California, Los Angeles, CA, USA 90095.ORCID 0000-0002-3519-7224

Kaustabh GhoshDepartment of Ophthalmology, University of California, Los Angeles, CA, USA 90095.ORCID 0000-0001-8185-9180

Funding

Role of Retinal Capillary Stiffness in Diabetic RetinopathyR01EY028242 · NEI · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI GHOSH, KAUSTABH · 2017 to 2021

$1.9M

NEI NIH HHS R01 EY028242

6 · The paper itself

Abstract

Persistent neutrophil activation contributes to the development of diabetic vascular complications in many organs including the retina. Past studies have implicated abnormal molecular or biochemical cues in neutrophil activation. Here we show that neutrophils can also be mechanically activated through an increase in bone marrow (BM) stiffness that results from diabetes-induced overexpression of matrix collagens and their crosslinker lysyl oxidase (LOX). Administration of LOX inhibitor β-aminopropionitrile (BAPN) in diabetic mice concomitantly blocked BM matrix stiffening and neutrophil activation (superoxide generation and gp91

Indexed as

Bone marrowHydrogelLysyl oxidaseNeutrophilRetinal endothelial cellsStiffness

Identifiers

PMID40950022

DOI10.1101/2025.08.30.673184

PMCPMC12424689

What OpenQuestion holds

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.