ArticleJournal of biomedical optics2025
ALA-based photodynamic priming in murine skin increases blood flow and oxygenation.
Article in Journal of biomedical optics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Significance: Topical photodynamic therapy (PDT) with protoporphyrin IX (PpIX) converted from 5-aminolevulinic acid (ALA) is a well-established noninvasive method of treating skin conditions and lesions. During PDT, there can be response dynamics within the tissue that are affected by the light delivery, seen with fractionated delivery and in subcurative priming delivery. Fractionated light doses can considerably increase efficacy of 5-ALA PDT response. Aim: We aim to examine the changes in physiological blood flow, tissue oxygenation, and PpIX concentration during and after light delivery in topical ALA-PDT in nude mouse skin. Approach: We compared three schemes of light delivery for topical ALA-PDT in nude mice, including (1) full light delivery without fractionation, (2) two equal fractions (50% and 50%) of light separated by 2 h, and (3) a 5% light dose fractionation by 2 h prior to the main 95% light dose. Tissue oxygen imaging was assessed with the hypoxia signal from delayed fluorescence of PpIX itself within the tissue, as well as by confirmation with Oxyphor phosphorescence lifetime quenching imaging. Results: The results of blood flow imaging and hypoxia imaging from PpIX and oxygen imaging with Oxyphor each showed evidence of increased capillary flow and tissue oxygenation after the initial 5% light dose, increased at the side of irradiation. This increased capillary flow and tissue oxygenation are presumably from vasodilation and local capillary flow increase. PpIX replenishment occurs during the intervening dark period after the initial light delivery. Conclusion: These observations suggest that increasing oxygen and capillary flow combined with increased PpIX production together yield increased PDT efficiency, amplified by this initial light dose from a photodynamic optical priming event occurring 2 h prior to full PDT light delivery.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.