Evidence map›Paper›PMID 40949421›Full record

ArticleBiochemistry and biophysics reports2025

A modified protocol for the isolation and culture of human umbilical vein endothelial cells.

Anmol Sandhu, Anannya Parvathi, Jennifer Jane McGhee, Salim Ismail, I-Ping Loh, Bert van der Werf, Jie Zhang, Trevor Sherwin

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In one paragraph

Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Anmol SandhuDepartment of Ophthalmology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
Anannya ParvathiDepartment of Ophthalmology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
Jennifer Jane McGheeDepartment of Ophthalmology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
Salim IsmailDepartment of Ophthalmology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
I-Ping LohDepartment of Ophthalmology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
Bert van der WerfDepartment of Epidemiology and Biostatistics, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
Jie ZhangDepartment of Ophthalmology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.
Trevor SherwinDepartment of Ophthalmology, Faculty of Medical and Health Sciences, The University of Auckland, Auckland, New Zealand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The umbilical cord is a valuable source of foetal stem cells, progenitor cells, and early-stage developmental cells, including human umbilical vein endothelial cells (HUVECs). HUVECs are widely used as a model for endothelial biology and are increasingly being investigated for their regenerative potential. Efficient isolation of these cells from the umbilical vein is a critical first step for both research and therapeutic applications. To date, most published protocols utilise Collagenase A for isolation. In this study, we present a modified HUVEC isolation protocol that employs dispase, alongside refined tissue and cell culture handling practices. We characterised the isolated cells using established HUVEC markers CD31 and CD146, and demonstrated in situ detachment of the cells from the vessel wall through immunofluorescence imaging. Our method achieved a success rate exceeding 95.6 % across all umbilical cords processed. These findings highlight the protocol's potential for broad applicability across research settings, using readily accessible reagents and equipment.

Indexed as

Cell isolation protocolEndothelial cellsEndotheliumHuman umbilical vein endothelial cellsHUVECsUmbilical cordVein

Identifiers

PMID40949421
PMCPMC12423704

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.