ArticleBiochemistry and biophysics reports2025
Identification of bile powder based on component data matrix and progressive matching strategy combined with UHPLC-QTOF-MS.
Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Background: Pig bile powder (PBP), chicken bile powder (CKBP), goat bile powder (GBP), bear bile powder (BBP), cow bile powder (CBP), rabbit bile powder (RBP) and snake bile powder (SBP) are commonly used bile powder medicinal materials in the clinic. Due to frequent confusion and fraudulent use, it is necessary to establish and enrich identification methods. In this paper, the component data matrix (CDM) contained common component matrix (CCM) and specificity component matrix (SCM), and progressive matching strategy (PMS) were innovatively proposed to identify PBP, CKBP, GBP, BBP, CBP, RBP and SBP. Methods: The samples were analyzed by LC-MS to obtain MS data on chemical components. Then, the CCM and SCM of each bile powder was obtained by taking the intersection and de-intersection of MS data. Finally, the CCM and SCM were used for matching test samples to obtain matching value (MV). At the same time, the proprietary components were further explored. Results: Based on CDM and PMS, the identification of PBP, CKBP, GBP, BBP, CBP, RBP and SBP can be quickly realized with MV ≥ 70 %.
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