ArticleFrontiers in pharmacology2025
Deciphering the role of myofibroblasts in microvascular invasion of hepatocellular carcinoma.
Article in Frontiers in pharmacology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Hepatocellular carcinoma (HCC) often exhibits microvascular invasion (MVI), a feature with unclear mechanisms. Therefore, it is crucial to resolve its related cellular populations and molecular networks using single-cell analysis. Methods: Both single-cell RNA sequencing (scRNA-seq) and RNA-seq data for HCC were obtained from public databases. ScRNA-seq data were clustered and annotated using Seurat. DAVID, CellChat, and Monocle 2 were used for scRNA-seq functional enrichment analysis, intercellular communication analysis, and cell trajectory analysis, respectively. We further assessed key gene expression in HCC cell lines and examined their effects on cell functions using CCK-8, scratch, and transwell assays. Results: The HCC ecosystem comprising myofibroblasts (MFs), hepatocytes, proliferative hepatocytes, endothelial cells, dendritic cells, proliferative NK/T cells, plasma B cells, and macrophages was revealed. MFs showed the greatest difference between MVI-absent and MVI-present patients and were subdivided into five clusters. Key genes for angiogenesis are overexpressed in MF2 cells and enriched in the pathways of angiogenesis, cell migration, cell proliferation, and signal transduction. Pseudotime analysis revealed MF2 cells from MVI-present patients clustered at the terminal state and positively correlated with angiogenesis. Conclusion: This study highlighted the role and potential mechanism of MFs in promoting MVI formation and provides a potential marker for HCC prognosis among MF markers.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.