Evidence map›Paper›PMID 40948894›Full record

ArticleBio-protocol2025

Simultaneous RNA Fluorescent In Situ Hybridization and Immunofluorescent Staining of Mouse Muscle Stem Cells on Fresh Frozen Skeletal Muscle Sections.

Vedant R Lakkundi, Maria L Perez, Kartik Soni, Albert E Almada

Abstract read
In one paragraph

Article in Bio-protocol, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Vedant R LakkundiDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA, USA.
Maria L PerezDepartment of Stem Cell Biology and Regenerative Medicine (SCRM), Keck School of Medicine, University of Southern California (USC), Los Angeles, CA, USA.
Kartik SoniDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA, USA.
Albert E AlmadaDepartment of Orthopaedic Surgery, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA, USA.

Funding

Stem Cell Dysfunction in Aged Skeletal MuscleR01AR080753 · NIAMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Albert Ernesto Almada · 2023 to 2026
$2.2M
NIAMS NIH HHS R01 AR080753
6 · The paper itself

Abstract

Adult muscle stem cells (MuSCs) are the key cellular source for regenerating skeletal muscle in vertebrates. MuSCs are typically identified in skeletal muscle by the expression of the paired box protein 7 (PAX7) protein. Here, we developed a combined RNA fluorescent in situ hybridization (FISH) using RNAscope technology and an immunofluorescence (IF) protocol for the simultaneous detection of

Indexed as

Fluorescent in situ hybridization (FISH)Immunofluorescence (IF)Muscle stem cellsPAX7RNAscopeSkeletal muscle

Identifiers

PMID40948894
PMCPMC12423284

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.