Evidence map›Paper›PMID 40947650›Full record

ArticleACS synthetic biology2025

Reconstitution of Human Cytochrome P450 Activity using a

Wayne A Johnston, Raine E S Thomson, Juan Alfaro-Palma, Robert E Speight, Kirill Alexandrov, Elizabeth M J Gillam, James B Y Behrendorff

Abstract read
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In one paragraph

Article in ACS synthetic biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Wayne A JohnstonCentre for Agriculture and the Bioeconomy, School of Biology and Environmental Science, Queensland University of Technology, Brisbane QLD 4000, Australia.
Raine E S ThomsonCentre for Agriculture and the Bioeconomy, School of Biology and Environmental Science, Queensland University of Technology, Brisbane QLD 4000, Australia.
Juan Alfaro-PalmaCentre for Agriculture and the Bioeconomy, School of Biology and Environmental Science, Queensland University of Technology, Brisbane QLD 4000, Australia.
Robert E SpeightAustralian Research Council Centre of Excellence in Synthetic Biology, Queensland University of Technology, Brisbane QLD 4000, Australia.
Kirill AlexandrovCentre for Agriculture and the Bioeconomy, School of Biology and Environmental Science, Queensland University of Technology, Brisbane QLD 4000, Australia.
Elizabeth M J GillamSchool of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane QLD 4072, Australia.ORCID 0000-0003-0378-793X
James B Y BehrendorffCentre for Agriculture and the Bioeconomy, School of Biology and Environmental Science, Queensland University of Technology, Brisbane QLD 4000, Australia.ORCID 0000-0003-4130-6252

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cytochrome P450 enzymes (P450s) are ubiquitous in drug metabolism and natural product biosynthesis. Studying eukaryotic P450s has been limited by their dependence on membrane association and the requirement for partner reductases. Here, we demonstrate cell-free synthesis and assay of human P450s 3A4 and 2D6 using

Indexed as

Cytochrome P-450 CYP2D6Cytochrome P-450 CYP3ACytochrome P-450 Enzyme SystemLeishmaniaArabidopsisCell-Free SystemHumansNADPH-Ferrihemoprotein ReductaseCYP3A4 protein, humanCytochrome P-450 CYP2D6Cytochrome P-450 CYP3ACytochrome P-450 Enzyme SystemNADPH-Ferrihemoprotein Reductasecell-free protein synthesiscytochrome P450cytochrome P450 reductaseLeishmania tarentolae

Identifiers

PMID40947650
PMCPMC12539551

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.