Evidence map›Paper›PMID 40947454›Full record

ArticleClinical oral investigations2025

Physicochemical properties and periodontal ligament stem cell response to NeoMTA 2.

Danilo Cassiano Ferraz, Jáder Camilo Pinto, Ariadne Letra, Renato Menezes Silva, Letícia Chaves de Souza, Juliane Maria Guerreiro-Tanomaru, Mario Tanomaru-Filho

Abstract read
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In one paragraph

Article in Clinical oral investigations, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Danilo Cassiano FerrazDepartment of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil. daniloferraz@pitt.edu.ORCID http://orcid.org/0000-0001-9102-4320
Jáder Camilo PintoDepartment of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.ORCID http://orcid.org/0000-0003-2023-1589
Ariadne LetraDepartment of Endodontics, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA.ORCID http://orcid.org/0000-0002-7197-6735
Renato Menezes SilvaDepartment of Endodontics, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA.ORCID http://orcid.org/0000-0002-6617-173X
Letícia Chaves de SouzaDepartment of Endodontics, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, USA.ORCID http://orcid.org/0000-0002-7057-9850
Juliane Maria Guerreiro-TanomaruDepartment of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.ORCID http://orcid.org/0000-0003-0446-2037
Mario Tanomaru-FilhoDepartment of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil.ORCID http://orcid.org/0000-0002-2574-4706

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001Fundação de Amparo à Pesquisa do Estado de São Paulo 2020/11476-0
6 · The paper itself

Abstract

objectivesTo investigate the physicochemical properties and cellular response of NeoMTA 2 as an endodontic sealer (NMTA2S) and root repair material (NMTA2R) compared to BioRoot RCS (BIR) and MTA Repair HP (MTAHP). MATERIALS AND

methodsSetting time, flow, radiopacity, and solubility were evaluated based on ISO 6876/2012 standards. Volumetric change was measured using micro-computed tomography. Periodontal ligament stem cells (PDLSCs) were used for cell viability and messenger RNA expression assays.

resultsNMTA2S had longer setting time than BIR (p < .05), while NMTA2R set longer than MTAHP (p < .05). All materials met ISO 6876 standards for radiopacity and flow, with similar solubility between NMTA2S and BIR, or NMTA2R and MTAHP (p > .05). NMTA2S decreased in volume, while BIR increased (p < .05). Volume changes were similar among root repair materials and immersion media (p > .05). All materials supported PDLSC viability. TNF was upregulated only in NMTA2S with the 6-hour eluate. COL1A1 and SPP1 were upregulated in all groups exposed to the 6-hour eluate. With the 24-hour eluate, only BIR sustained COL1A1 signaling, and did not significantly express SPP1. With the 48-hour eluate, COL1A1 was upregulated in all groups along with ALPL.

conclusionDespite their longer setting times, NMTA2S and NMTA2R exhibited acceptable physicochemical and biological properties. Additionally, NMTA2S and NMTA2R appear to offer distinct advantages towards an osteogenic potential, which provides valuable insights for endodontic or reparative treatment choices. CLINICAL RELEVANCE: This study provides the first evidence of osteogenic gene expression in PDLSCs by NeoMTA 2, as an endodontic sealer and root repair material. The findings highlight NeoMTA 2's distinct biological response at different eluate time points, reinforcing its potential for endodontic and reparative applications.

Indexed as

Aluminum CompoundsCalcium CompoundsOxidesPeriodontal LigamentRoot Canal Filling MaterialsSilicatesStem CellsCells, CulturedCell SurvivalDrug CombinationsHumansMaterials TestingSolubilityX-Ray MicrotomographyAluminum CompoundsCalcium CompoundsDrug Combinationsmineral trioxide aggregateOxidesRoot Canal Filling MaterialsSilicatesCalcium silicateEndodonticsGene expression profilingX-Ray microtomography

Identifiers

PMID40947454

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.