Evidence map›Paper›PMID 40946956›Full record

ArticleEuropean journal of pharmacology2025

Mutational effects of the asparagine198 and glutamate223 residues on the human norepinephrine transporter on basal and HIV-1 Tat protein-induced inhibition of dopamine transport.

Katherine Darby Porter, Charles Adeniran, Ana Catya Jimenez-Torres, Karl Lee Pless, Abagail Brenda Cirincione, Harper Davenport, Carolyn Chen, Chang-Guo Zhan, Jun Zhu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in European journal of pharmacology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. EcoHIV Infection Disrupts Dopamine and Serotonin Transporter Function, Altering Release Dynamics in C57BL/6J Mice.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Katherine Darby PorterDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, SC, USA.
Charles AdeniranMolecular Modeling and Biopharmaceutical Center, University of Kentucky, Lexington, KY, USA; Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY, USA.
Ana Catya Jimenez-TorresDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, SC, USA; Department of Pharmacology and Toxicology, Medical College of Gerogia, Augusta University, Augusta, GA, USA.
Karl Lee PlessDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, SC, USA.
Abagail Brenda CirincioneDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, SC, USA.
Harper DavenportDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, SC, USA.
Carolyn ChenDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, SC, USA.
Chang-Guo ZhanMolecular Modeling and Biopharmaceutical Center, University of Kentucky, Lexington, KY, USA; Department of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY, USA.
Jun ZhuDepartment of Drug Discovery and Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, SC, USA; Department of Pharmacology and Toxicology, Medical College of Gerogia, Augusta University, Augusta, GA, USA. Electronic address: JUZHU@Augusta.edu.

Funding

Molecular mechanisms: Dysregulation of monoamine transporters by HIV-1Tat and cocaineR01DA035714 · NIDA · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · PI Jay P. McLaughlin, CHANG-GUO ZHAN · 2013 to 2026
$6.6M
NIDA NIH HHS R01 DA035714
6 · The paper itself

Abstract

HIV-1 transactivator of transcription (Tat) protein induces dopaminergic dysregulation, which plays a central role in HIV-1-associated neurocognitive disorders. Computational modeling predicts that asparagine 198 and glutamate 223 of the human norepinephrine transporter (hNET) are key residues involved in Tat binding. This study investigated the effects of N198A and E223A mutations on basal and Tat-induced inhibition of dopamine (DA) uptake via hNET in CHO cells expressing WT hNET or its mutants. Compared to WT hNET, E223A mutation increased the affinity for nisoxetine and cocaine in inhibiting [

Indexed as

AsparagineDopamineGlutamic AcidHIV-1MutationNorepinephrine Plasma Membrane Transport Proteinstat Gene Products, Human Immunodeficiency VirusAnimalsBiological TransportCHO CellsCocaineCricetulusFluoxetineHumansAsparagineCocaineDopamineFluoxetineGlutamic AcidnisoxetineNorepinephrine Plasma Membrane Transport Proteinstat Gene Products, Human Immunodeficiency VirusConformational transitionsDopamineHIVNorepinephrine transporterUptake

Identifiers

PMID40946956
PMCPMC13046434

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.