Evidence map›Paper›PMID 40946256›Full record

ArticleEuropean biophysics journal : EBJ2025

Mitochondrial alterations induced by Macrovipera lebetina venom.

Virjinia Doltchinkova, Victoria Vitkova, Milena Shkodrova, Meglena Kitanova, Vessela Lozanova, Siya Lozanova, Avgust Ivanov, Chavdar Roumenin

Abstract read
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In one paragraph

Article in European biophysics journal : EBJ, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Virjinia DoltchinkovaInstitute of Robotics "Saint Apostle and Gospeller Mathew", Bulgarian Academy of Sciences, (IR-BAS), Acad. Georgi Bonchev Str., Bl. 2, P.O. Box 79, 1113, Sofia, Bulgaria. vdoltchinkova@gmail.com.ORCID http://orcid.org/0000-0003-3096-7311
Victoria VitkovaGeorgi Nadjakov Institute of Solid State Physics, Bulgarian Academy of Sciences, 72 Tsarigradsko Chaussee Blvd., 1784, Sofia, Bulgaria. victoria@issp.bas.bg.
Milena ShkodrovaLaboratory of Cell Bioenergetics, Department of Human and Animal Physiology, Faculty of Biology, Sofia University "St. Kliment Ohridski", 8 Dragan Tzankov Blvd., 1164, Sofia, Bulgaria.
Meglena KitanovaDepartment of Genetics, Faculty of Biology, Sofia University "St. Kliment Ohridski", 8 Dragan Tzankov Blvd., 1164, Sofia, Bulgaria.
Vessela LozanovaDepartment of Medical Chemistry and Biochemistry, Medical Faculty, Medical University, 2 Zdrave Str., 1431, Sofia, Bulgaria.
Siya LozanovaInstitute of Robotics "Saint Apostle and Gospeller Mathew", Bulgarian Academy of Sciences, (IR-BAS), Acad. Georgi Bonchev Str., Bl. 2, P.O. Box 79, 1113, Sofia, Bulgaria.
Avgust IvanovInstitute of Robotics "Saint Apostle and Gospeller Mathew", Bulgarian Academy of Sciences, (IR-BAS), Acad. Georgi Bonchev Str., Bl. 2, P.O. Box 79, 1113, Sofia, Bulgaria.
Chavdar RoumeninInstitute of Robotics "Saint Apostle and Gospeller Mathew", Bulgarian Academy of Sciences, (IR-BAS), Acad. Georgi Bonchev Str., Bl. 2, P.O. Box 79, 1113, Sofia, Bulgaria.

Funding

Bulgarian National Science Fund KP-06-N38/14/2019
6 · The paper itself

Abstract

The development of snake venom-based therapeutics and antivenoms is closely linked to understanding how snake venoms modulate the physicochemical properties of biological membranes. In this study, we investigated the effects of Macrovipera lebetina venom on the surface electrical characteristics of rat liver mitochondrial membranes in vitro. A combination of electrokinetic and mechanical techniques was employed, including measurements of mitochondrial electrophoretic mobility and the bending elasticity of phosphatidylcholine membrane models. Exposure to Macrovipera lebetina venom significantly increased the net surface charge of mitochondria, while adenosine triphosphatase (ATPase) activity remained unchanged. Morphological alterations and vesicle aggregation, observed via light scattering and fluorescence microscopy, indicated reduced binding of α-D-mannosyl and α-D-glucosyl residues to the outer mitochondrial membrane in the presence of venom. In addition, malondialdehyde assays revealed elevated levels of oxidized lipid species in venom-treated mitochondria. A pronounced softening of model phosphatidylcholine membranes was also observed, likely associated with lipid oxidation induced by the venom. It states that the effect of the venom of the Macrovipera lebetina on the surface electrical charge of rat liver mitochondria is being studied in vitro. The surface charge is a physicochemical characteristic of the mitochondrial membrane.

Indexed as

Mitochondria, LiverViper VenomsAdenosine TriphosphatasesAnimalsMaleMitochondrial MembranesPhosphatidylcholinesRatsRats, WistarViperidaeAdenosine TriphosphatasesPhosphatidylcholinesViper VenomsATPase activityLipid peroxidationMacrovipera lebetina venomMembrane elasticityMitochondriaSurface charge

Identifiers

PMID40946256

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.