Evidence map›Paper›PMID 40945605›Full record

ArticleBrain stimulation

Photoswitching endogenous glutamate receptors in neural ensembles and single synapses in vivo.

Aida Garrido-Charles, Miquel Bosch, Hyojung Lee, Xavier Rovira, Silvia Pittolo, Artur Llobet, Hovy Ho-Wai Wong, Ana Trapero, Carlo Matera, Claudio Papotto and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain stimulation. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Assessing circuit function in the developingFrontiers in behavioral neuroscience · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Aida Garrido-CharlesInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), Carrer de Baldiri Reixac 15-21, 08028, Barcelona, Spain; Network Biomedical Research Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029, Madrid, Spain; Institute for Cardiovascular Physiology, University Medical Center Göttingen, Humboldtallee 23, 37073, Göttingen, Germany.
Miquel BoschInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), Carrer de Baldiri Reixac 15-21, 08028, Barcelona, Spain; Network Biomedical Research Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029, Madrid, Spain; Department of Biomedical Sciences, Universitat Internacional de Catalunya (UIC), 08195, Sant Cugat del Vallès, Spain.
Hyojung LeeInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), Carrer de Baldiri Reixac 15-21, 08028, Barcelona, Spain; Network Biomedical Research Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029, Madrid, Spain.
Xavier RoviraInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), Carrer de Baldiri Reixac 15-21, 08028, Barcelona, Spain; Network Biomedical Research Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029, Madrid, Spain.
Silvia PittoloInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), Carrer de Baldiri Reixac 15-21, 08028, Barcelona, Spain; Network Biomedical Research Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029, Madrid, Spain.
Artur LlobetLaboratory of Neurobiology, Neuroscience Program, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Spain; Department of Pathology and Experimental Therapy, School of Medicine, University of Barcelona, L'Hospitalet de Llobregat, Spain; Institute of Neurosciences, University of Barcelona, L'Hospitalet de Llobregat, Spain.
Hovy Ho-Wai WongDepartment of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK; Gerald Choa Neuroscience Institute & School of Biomedical Sciences, The Chinese University of Hong Kong, Hong Kong, China.
Ana TraperoInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), Carrer de Baldiri Reixac 15-21, 08028, Barcelona, Spain; Institute of Advanced Chemistry of Catalonia, Consejo Superior de Investigaciones Científicas (IQAC-CSIC), 08034, Barcelona, Spain.
Carlo MateraInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), Carrer de Baldiri Reixac 15-21, 08028, Barcelona, Spain; Network Biomedical Research Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029, Madrid, Spain.
Claudio PapottoInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), Carrer de Baldiri Reixac 15-21, 08028, Barcelona, Spain.
Carme SerraInstitute of Advanced Chemistry of Catalonia, Consejo Superior de Investigaciones Científicas (IQAC-CSIC), 08034, Barcelona, Spain.
Amadeu LlebariaInstitute of Advanced Chemistry of Catalonia, Consejo Superior de Investigaciones Científicas (IQAC-CSIC), 08034, Barcelona, Spain.
Eduardo SorianoDepartment of Cell Biology, Physiology, and Immunology, Institute of Neurosciences, University of Barcelona (UB), 08028, Barcelona, Spain; Network Center of Biomedical Research in Neurodegenerative Diseases (CIBERNED), 28031, Madrid, Spain.
Maria V Sanchez-VivesInstitut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain; Catalan Institution for Research and Advanced Studies (ICREA), 08010, Barcelona, Spain.
Christine E HoltDepartment of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, UK.
Pau GorostizaInstitute for Bioengineering of Catalonia (IBEC), Barcelona Institute for Science and Technology (BIST), Carrer de Baldiri Reixac 15-21, 08028, Barcelona, Spain; Network Biomedical Research Center in Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN), 28029, Madrid, Spain; Catalan Institution for Research and Advanced Studies (ICREA), 08010, Barcelona, Spain. Electronic address: pau@icrea.cat.

Funding

Marie Curie H2020-MSCA-IF-2014
6 · The paper itself

Abstract

purposeTo interrogate animal physiology in vivo, there is a lack of non-genetic methods to control the activity of endogenous proteins with pharmacological and spatiotemporal precision. To address this need, we recently developed targeted covalent photoswitchable (TCP) compounds that enable the remote control of endogenous glutamate receptors (GluRs) using light.

methodsWe combine the photopharmacological effector TCP9 with neuronal activity sensors to demonstrate all-optical reversible control of endogenous GluRs across multiple spatiotemporal scales in rat brain tissue ex vivo and in Xenopus tadpole brains in vivo.

findingsTCP9 allows photoactivation of neuronal ensembles, individual neurons, and single synapses in ex vivo tissue and in intact brain in vivo, which is challenging using optogenetics and neurotransmitter uncaging. TCP9 covalently targets AMPA and kainate receptors, maintaining their functionality and photoswitchability for extended periods (>8 h) after a single compound application. This allows tracking endogenous receptor physiology during synaptic plasticity events such as the reduction of functional AMPA receptors during long-term depression in hippocampal neurons.

conclusionTCP9 is a unique non-invasive tool for durable labeling, reversible photoswitching, and functional tracking of native receptors in brain tissue without genetic manipulation.

Indexed as

BrainNeuronsOptogeneticsReceptors, AMPAReceptors, GlutamateSynapsesAnimalsHippocampusKainic Acid ReceptorsNeuronal PlasticityRatsXenopus laevisKainic Acid ReceptorsReceptors, AMPAReceptors, GlutamateAMPARAzobenzeneCalcium imagingCovalent drugDendritic spinesHippocampusKainateLong-term depressionOptopharmacologyPhotopharmacologyPhotoswitchPlasticityPulse-chaseRatXenopus

Identifiers

PMID40945605
PMCPMC12643953

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.