Evidence map›Paper›PMID 40945510›Full record

ArticleCurrent biology : CB2025

Slit2/Robo1 signaling constrains image stabilization responses to preserve ethologically favorable directional asymmetry.

James K Kiraly, Annika Balraj, Paige Leary, Zihao You, Scott C Harris, Jeanette D Hyer, Felice A Dunn, Alex L Kolodkin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current biology : CB, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

James K KiralySolomon H. Snyder Department of Neuroscience, The Johns Hopkins Kavli Neuroscience Discovery Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Annika BalrajDepartment of Ophthalmology, University of California, San Francisco, San Francisco, CA 94158, USA.
Paige LearyDepartment of Ophthalmology, University of California, San Francisco, San Francisco, CA 94158, USA.
Zihao YouSolomon H. Snyder Department of Neuroscience, The Johns Hopkins Kavli Neuroscience Discovery Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Scott C HarrisDepartment of Ophthalmology, University of California, San Francisco, San Francisco, CA 94158, USA.
Jeanette D HyerDepartment of Ophthalmology, University of California, San Francisco, San Francisco, CA 94158, USA.
Felice A DunnDepartment of Ophthalmology, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address: felice.dunn@ucsf.edu.
Alex L KolodkinSolomon H. Snyder Department of Neuroscience, The Johns Hopkins Kavli Neuroscience Discovery Institute, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: kolodkin@jhmi.edu.

Funding

Visual Science Training ProgramT32EY007143 · NEI · JOHNS HOPKINS UNIVERSITY · PI Laura Ensign, JEREMY NATHANS · 1995 to 2026
$6.2M
Molecular mechanisms underlying direction-selective circuit assembly and function in the mouse visual systemR01EY032095 · NEI · JOHNS HOPKINS UNIVERSITY · PI KOLODKIN, ALEX L · 2021 to 2025
$2.3M
Retinal mechanisms underlying the optokinetic reflexR01EY035028 · NEI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Felice A Dunn, ALEX L KOLODKIN · 2024 to 2026
$1.6M
Central mechanisms for integrating distinct retinal inputs during the optokinetic reflex in miceF31EY033225 · NEI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HARRIS, SCOTT C · 2021 to 2023
$121k
NEI NIH HHS F31 EY033225NEI NIH HHS R01 EY032095NEI NIH HHS R01 EY035028NEI NIH HHS T32 EY007143
6 · The paper itself

Abstract

Visual sensation relies on retinal circuitry to receive environmental inputs and convey relevant information for behavioral outputs. Many species depend on gaze stabilization behaviors, such as the optokinetic reflex (OKR), to perceive and correct for global motion. OKR calculation begins in the retina, where ON direction-selective ganglion cells (oDSGCs) respond to slow visual motion and deliver information to the accessory optic system (AOS) to inform oculomotor outputs. Here, we find that the guidance receptor roundabout-1 (Robo1) and its ligand Slit2 are selectively expressed in mammalian retinal AOS components and refine oDSGC circuitry to constrain OKR gain for appropriate image stabilization responses. Robo1 or Slit2 loss-of-function (LOF) mutants exhibit an increase in OKR gain, reducing directional asymmetry by elevating naturally weaker OKR responses. These behavioral enhancements largely arise from alterations in the retina, and loss of Slit2 in starburst amacrine cells (SACs) enhances oDSGC firing properties, with increased spike responses, excitatory synaptic puncta, and downstream AOS activation. These findings reveal that the Slit2/Robo1 signaling tunes oDSGC circuitry to maintain regulated image stabilization behavior.

Indexed as

Intercellular Signaling Peptides and ProteinsNerve Tissue ProteinsReceptors, ImmunologicSignal TransductionAmacrine CellsAnimalsMiceRetinaRetinal Ganglion CellsRoundabout ProteinsSlit Homolog 2 ProteinIntercellular Signaling Peptides and ProteinsNerve Tissue ProteinsReceptors, ImmunologicRoundabout ProteinsSlit Homolog 2 Proteinaccessory optic systemdirection selectivityneural developmentoptokinetic reflexretinaretinal ganglion cellsvisual system

Identifiers

PMID40945510
PMCPMC12553481

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.