Evidence map›Paper›PMID 40944454›Full record

ArticleProtein science : a publication of the Protein Society2025

The C-terminal extension of calprotectin mediates zinc chelation and modulates Staphylococcus aureus biomass accumulation.

Yasiru R Perera, Kyle T Enriquez, Aslin Rodriguez, Velia Garcia, Tae Akizuki, Anais Naretto, Melumo Togashi, Ryan Guillen, Eric P Skaar, Walter J Chazin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Microbiology spectrum · 2026
    Article
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yasiru R PereraDepartments of Biochemistry and Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Kyle T EnriquezVanderbilt University Medical Scientist Training Program, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Aslin RodriguezDepartments of Biochemistry and Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Velia GarciaDepartments of Biochemistry and Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Tae AkizukiDepartments of Biochemistry and Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Anais NarettoDepartments of Biochemistry and Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Melumo TogashiDepartments of Biochemistry and Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Ryan GuillenDepartments of Biochemistry and Chemistry, Vanderbilt University, Nashville, Tennessee, USA.
Eric P SkaarDepartment of Pathology, Microbiology, and Immunology, Vanderbilt University, Nashville, Tennessee, USA.
Walter J ChazinDepartments of Biochemistry and Chemistry, Vanderbilt University, Nashville, Tennessee, USA.ORCID 0000-0002-2180-0790

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007347 · NIGMS · VANDERBILT UNIVERSITY · PI WILLIAMS, CHRISTOPHER S. · 1985 to 2023
$26.3M
Host-mediated zinc sequestration during Acinetobacter baumannii infectionR01AI101171 · NIAID · VANDERBILT UNIVERSITY MEDICAL CENTER · PI WALTER J. CHAZIN, DAVID P. GIEDROC · 2013 to 2026
$8.2M
Neisseria gonorrhoeae metal transporters that subvert nutritional immunityR01AI127793 · NIAID · VIRGINIA COMMONWEALTH UNIVERSITY · PI CYNTHIA N CORNELISSEN, Alison K Criss · 2017 to 2026
$6.8M
Molecular mapping of microbial communities at the host-pathogen interface by multi-modal 3-dimensional imaging mass spectrometryR01AI138581 · NIAID · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Eric P Skaar, Jeffrey M Spraggins · 2018 to 2026
$5.2M
The Staphylococcus aureus response to nutrient zinc restriction during infectionR01AI150701 · NIAID · VANDERBILT UNIVERSITY MEDICAL CENTER · PI SKAAR, ERIC P · 2020 to 2024
$2.1M
S. aureus COG0523s as bacterial zinc metallochaperones critical to pathogenesisF30AI181344 · NIAID · VANDERBILT UNIVERSITY · PI Kyle T Enriquez · 2024 to 2026
$144k
NIAID NIH HHS F30 AI181344NIAID NIH HHS R01 AI101171NIAID NIH HHS R01 AI127793NIAID NIH HHS R01 AI138581NIAID NIH HHS R01 AI150701NIGMS NIH HHS T32 GM007347NIH HHS F30AI181344NIH HHS R01145992NIH HHS R01 AI101171NIH HHS R01 AI127793NIH HHS R01AI138581NIH HHS R01AI150701NIH HHS T32GM007347
6 · The paper itself

Abstract

Calprotectin (CP) is an S100A8/S100A9 heterodimer that plays an important role in nutritional immunity at the host-microbe interface. CP combats Staphylococcus aureus growth by sequestration of zinc and other trace transition metals; however, questions remain about whether CP antimicrobial activity strictly relies on metal sequestration. Moreover, the precise mechanism for how zinc binds at the two distinct transition metal binding sites of CP is not known. High-resolution X-ray crystal structures reveal tetracoordinate binding in the canonical His

Indexed as

Leukocyte L1 Antigen ComplexStaphylococcus aureusZincBinding SitesBiofilmsBiomassCrystallography, X-RayHumansModels, MolecularLeukocyte L1 Antigen ComplexZincbiofilmcalprotectinnutritional immunityStaphylococcus aureusX‐ray crystallographyzinc

Identifiers

PMID40944454
PMCPMC12432413

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.