ArticleAnimals : an open access journal from MDPI2025
Fetal Bovine Serum Supplementation Enhances Functional Consistency of IGRA Results in Bovine Tuberculosis Diagnostics.
Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Development of a dual fluorescent microsphere-based lateral flow immunoassays for simultaneous detection ofFrontiers in veterinary science · 2026Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The interferon-gamma release assay (IGRA) is a reliable diagnostic tool for bovine tuberculosis (bTB) due to its high sensitivity and specificity. However, the assay relies on viable T-cell function, making it susceptible to functionally undetectable responses during sample storage. This study aimed to evaluate whether fetal bovine serum (FBS) supplementation could mitigate functional deterioration and stabilize immune responses in stored blood samples. The IGRA was conducted on blood samples from 91 cattle under three conditions: fresh (Day 0), stored without FBS (FBS X), and stored with 10% FBS (FBS O). A dual stimulation using bovine PPD (bovis) and mitogen revealed that the FBS O condition significantly preserved IFN-γ responses, with a higher frequency of simultaneous bovis and mitogen recovery (dual recovery). Additional correlation analysis between MTT cell viability and mitogen response further suggested that FBS contributes to T-cell functionality beyond survival. These findings suggest that FBS supplementation improves the functional consistency of IGRA results and reduces the risk of functionally undetectable responses in delayed testing scenarios.
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