Reducing Heat Without Impacting Quality: Optimizing Trypsin Inhibitor Inactivation Process in Low-TI Soybean.

A soybean meal is a key protein source in human foods and animal feed, yet its digestibility is constrained by endogenous trypsin inhibitors (TIs). Thermal processing is the mainstream tool for TI inactivation, but high-intensity heat treatments increase energy consumption and can potentially denature proteins, diminishing nutritional quality. Reducing the thermal input while maintaining nutritional quality is, therefore, a critical challenge. One promising strategy is the use of soybean cultivars bred for low-TI expression, which may allow for milder processing. However, the performance of these low-TI cultivars under reduced heat conditions remains unstudied. This study treated soybean samples under four different temperatures (60, 80, 100, and 121 °C) for 10 min and investigated the impact of heat treatment on TI concentration, in vitro protein digestibility, and nutritional properties of meals from a conventional high-TI variety (Glenn) and a novel low-TI variety (VT Barrack). Results showed that heat treatment at 100 °C significantly improved protein digestibility and lower TI concentrations in both varieties. A negative correlation was observed between protein digestibility and TI concentration in both soybean varieties. At 100 °C, the low-TI variety achieved 81.4% protein digestibility with only 0.6 mg/g TIs, whereas the high-TI variety required 121 °C to achieve comparable protein digestibility and a TI reduction. These findings highlight that low-TI soybeans can lower the necessary thermal treatment to 100 °C to minimize TIs while simultaneously preserving protein quality and cutting energy demand, offering a practical, cost-effective approach to producing higher-quality soybean meals.