Evidence map›Paper›PMID 40940722›Full record

ArticleCells2025

Generation and Characterization of Cisplatin-Resistant Oral Squamous Cell Carcinoma Cells Displaying an Epithelial-Mesenchymal Transition Signature.

Everton Freitas de Morais, Lilianny Querino Rocha de Oliveira, Cintia Eliza Marques, Fábio Haach Téo, Gisele Vieira Rocha, Camila Oliveira Rodini, Clarissa A Gurgel, Tuula Salo, Edgard Graner, Ricardo D Coletta

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Review
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  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Everton Freitas de MoraisGraduate Program in Oral Biology, School of Dentistry, University of Campinas, Piracicaba 13414-018, SP, Brazil.ORCID 0000-0002-2173-7672
Lilianny Querino Rocha de OliveiraGraduate Program in Oral Biology, School of Dentistry, University of Campinas, Piracicaba 13414-018, SP, Brazil.
Cintia Eliza MarquesGraduate Program in Oral Biology, School of Dentistry, University of Campinas, Piracicaba 13414-018, SP, Brazil.
Fábio Haach TéoDepartment of Oral Diagnosis, School of Dentistry, University of Campinas, Piracicaba 13414-018, SP, Brazil.
Gisele Vieira RochaGonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador 40296-710, BA, Brazil.
Camila Oliveira RodiniDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru 05508-220, SP, Brazil.
Clarissa A GurgelGonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA), Salvador 40296-710, BA, Brazil.ORCID 0000-0001-8922-2985
Tuula SaloCancer and Translational Medicine Research Unit, Faculty of Medicine and Medical Research Center Oulu, Oulu University Hospital, University of Oulu, 90014 Oulu, Finland.
Edgard GranerDepartment of Oral Diagnosis, School of Dentistry, University of Campinas, Piracicaba 13414-018, SP, Brazil.
Ricardo D ColettaGraduate Program in Oral Biology, School of Dentistry, University of Campinas, Piracicaba 13414-018, SP, Brazil.ORCID 0000-0001-5285-3046

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2022/00994-5Fundação de Amparo à Pesquisa do Estado de São Paulo 2022/08908-0
6 · The paper itself

Abstract

Cisplatin resistance remains a major therapeutic challenge in oral squamous cell carcinoma (OSCC), leading to treatment failure and poor outcomes. This study aimed to generate and characterize cisplatin-resistant OSCC models to elucidate resistance mechanisms. Two resistant OSCC cell lines (SCC-9R and HSC-3R) were developed through gradual dose escalation. Parental and resistant cells were analyzed via RNA-seq and gene set enrichment analysis, and validated through RT-qPCR, Western blot, immunofluorescence, and gelatin zymography. Functional assays, including 2D and 3D migration and invasion models, assessed phenotypic changes. A multi-omics analysis revealed molecular alterations in resistant cells, including 305 differentially expressed genes (DEGs) in HSC-3R (187 upregulated) and 782 in SCC-9R (298 upregulated) versus parental lines, with enrichment for extracellular matrix organization (

Indexed as

Carcinoma, Squamous CellCisplatinDrug Resistance, NeoplasmEpithelial-Mesenchymal TransitionMouth NeoplasmsCell Line, TumorCell MovementGene Expression Regulation, NeoplasticHumansCisplatinbiomarkerscisplatin resistanceepithelial–mesenchymal transitionoral squamous cell carcinomatumor plasticity

Identifiers

PMID40940722
PMCPMC12427644

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.