Evidence map›Paper›PMID 40940337›Full record

ArticleNature communications2025

Matrix directs trophoblast differentiation in a bioprinted organoid model of early placental development.

Claire Richards, Hao Chen, Matthew O'Rourke, Ashley Bannister, Grace Owen, Alexander Volkerling, Arnab Ghosh, Catherine A Gorrie, David Gallego-Ortega, Amy L Bottomley and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Maternal-fetal interface organoids: a new era in pregnancy research.Frontiers in bioengineering and biotechnology · 2026
    Review
  3. Beyond monolayers: a comparative analysis of 2D cell cultures and 3DFrontiers in bioengineering and biotechnology · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Claire RichardsSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.ORCID http://orcid.org/0000-0001-5080-9164
Hao ChenCentre for Inflammation, Centenary Institute and University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW, Australia.
Matthew O'RourkeCentre for Inflammation, Centenary Institute and University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW, Australia.
Ashley BannisterSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
Grace OwenSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
Alexander VolkerlingInventia Life Science Pty Ltd, Sydney, NSW, Australia.
Arnab GhoshSchool of Biomedical Sciences and Pharmacy, University of Newcastle, Callaghan, University of Newcastle, Callaghan, Newcastle, NSW, Australia.
Catherine A GorrieSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
David Gallego-OrtegaSingle Cell Genomics Facility, School of Biomedical Engineering, Faculty of Engineering and Information Technology, University of Technology Sydney, Sydney, NSW, Australia.ORCID http://orcid.org/0000-0002-2347-7835
Amy L BottomleyMicrobial Imaging Facility at the Australian Institute for Microbial Imaging, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.ORCID http://orcid.org/0000-0003-0255-0869
Matthew P PadulaSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.ORCID http://orcid.org/0000-0002-8283-0643
Kristine C McGrathSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.ORCID http://orcid.org/0000-0002-6244-3929
Louise ColeMicrobial Imaging Facility at the Australian Institute for Microbial Imaging, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia.
Philip M HansbroCentre for Inflammation, Centenary Institute and University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW, Australia.ORCID http://orcid.org/0000-0002-4741-3035
Lana McClementsSchool of Life Sciences, Faculty of Science, University of Technology Sydney, Sydney, NSW, Australia. lana.mcclements@uts.edu.au.ORCID http://orcid.org/0000-0002-4911-1014

Funding

National Heart Foundation of Australia (Heart Foundation) 106628
6 · The paper itself

Abstract

Trophoblast organoids can provide crucial insights into mechanisms of placentation, however their potential is limited by highly variable extracellular matrices unable to reflect in vivo tissues. Here, we present a bioprinted placental organoid model, generated using the first trimester trophoblast cell line, ACH-3P, and a synthetic polyethylene glycol (PEG) matrix. Bioprinted or Matrigel-embedded organoids differentiate spontaneously from cytotrophoblasts into two major subtypes: extravillous trophoblasts (EVTs) and syncytiotrophoblasts (STBs). Bioprinted organoids are driven towards EVT differentiation and show close similarity with early human placenta or primary trophoblast organoids. Inflammation inhibits proliferation and STBs within bioprinted organoids, which aspirin or metformin (0.5 mM) cannot rescue. We reverse the inside-out architecture of ACH-3P organoids by suspension culture with STBs forming on the outer layer of organoids, reflecting placental tissue. Our bioprinted methodology is applicable to trophoblast stem cells. We present a high-throughput, automated, and tuneable trophoblast organoid model that reproducibly mimics the placental microenvironment in health and disease.

Indexed as

BioprintingCell DifferentiationExtracellular MatrixOrganoidsPlacentationTrophoblastsCell LineCollagenDrug CombinationsFemaleHumansLamininModels, BiologicalPlacentaPolyethylene GlycolsPregnancyCollagenDrug CombinationsLamininmatrigelPolyethylene GlycolsProteoglycans

Identifiers

PMID40940337
PMCPMC12432263

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.