Evidence map›Paper›PMID 40939757›Full record

ArticleThe Journal of allergy and clinical immunology2025

Identification of disease-specific regulatory networks in prurigo nodularis versus atopic dermatitis through ATAC/RNA single-nucleus profiling.

Yuntian Wu, Rachael Bogle, Matthew T Patrick, Gharaee-Kermani Mehrnaz, Xianying Xing, Allison C Billi, J Michelle Kahlenberg, Valérie Julia, Johann E Gudjonsson, Lam C Tsoi

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In one paragraph

Article in The Journal of allergy and clinical immunology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Epithelial-Dermal Immune Memory: TrackingInternational journal of molecular sciences · 2026
    Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuntian WuDepartment of Biostatistics, University of Michigan, Ann Arbor, Mich.
Rachael BogleDepartment of Dermatology, University of Michigan Medical School, Ann Arbor, Mich.
Matthew T PatrickDepartment of Dermatology, University of Michigan Medical School, Ann Arbor, Mich.
Gharaee-Kermani MehrnazDepartment of Dermatology, University of Michigan Medical School, Ann Arbor, Mich.
Xianying XingDepartment of Internal Medicine, Division of Rheumatology, University of Michigan, Ann Arbor, Mich.
Allison C BilliDepartment of Dermatology, University of Michigan Medical School, Ann Arbor, Mich.
J Michelle KahlenbergDepartment of Dermatology, University of Michigan Medical School, Ann Arbor, Mich; Department of Internal Medicine, Division of Rheumatology, University of Michigan, Ann Arbor, Mich.
Valérie JuliaGalderma, Zug, Switzerland.
Johann E GudjonssonMary H. Weiser Food Allergy Center, University of Michigan, Ann Arbor, Mich; Department of Dermatology, University of Michigan Medical School, Ann Arbor, Mich. Electronic address: johanng@med.umich.edu.
Lam C TsoiDepartment of Biostatistics, University of Michigan, Ann Arbor, Mich; Department of Dermatology, University of Michigan Medical School, Ann Arbor, Mich; Mary H. Weiser Food Allergy Center, University of Michigan, Ann Arbor, Mich; Department of Computational Medicine & Bioinformatics, University of Michigan, Ann Arbor, Mich. Electronic address: alextsoi@med.umich.edu.

Funding

Immunogenomics and Systems Biology CoreUC2AR081033 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Johann Eli Gudjonsson, Lam Cheung Tsoi · 2022 to 2026
$3.3M
Integrative and trans-ethnic study to understand psoriasis associated signalsR01AR080662 · NIAMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Lam Cheung Tsoi · 2023 to 2026
$1.4M
NIAMS NIH HHS R01 AR080662NIAMS NIH HHS UC2 AR081033
6 · The paper itself

Abstract

backgroundPrurigo nodularis (PN) is a chronic neuroimmune skin disorder characterized by chronic itch and multiple pruriginous nodules, and is related to atopic dermatitis (AD). While different transcriptomic studies have been conducted, the regulatory mechanisms and differences between PN and AD remain poorly understood.

objectiveWe aimed to uncover shared and distinct regulatory mechanisms between PN and AD. Specifically, we evaluated how molecular factors driving the distinction between lesional and nonlesional skin differ in these two diseases.

methodWe profiled gene expression and chromatin accessibility at the single-nucleus level from paired lesional and nonlesional skin samples of 4 PN patients and 5 AD patients. We utilized joint modeling and pseudobulk techniques to identify disease specific regulatory network.

resultsThe epigenomic alterations in PN were more numerous and of greater magnitude compared to AD skin. PN-biased cis-regulatory elements showed correlation with PN-biased differentially expressed genes in the same cell type, and we identified 199 regulatory modules specific to PN, in contrast to 3 modules specific to AD. We also identified PN-specific AP-1-induced regulatory module that induces RUNX2 in fibroblasts, confirmed by immunohistochemistry and immunofluorescence staining, suggestive of its contribution to fibrosis in PN.

conclusionThis study provides a chromatin RNA expression atlas and underscores its efficacy in revealing distinct regulatory mechanisms inherent to PN and AD.

Indexed as

Dermatitis, AtopicGene Regulatory NetworksPrurigoAdultChromatinFemaleGene Expression ProfilingHumansMaleMiddle AgedSkinTranscriptomeChromatinatopic dermatitislesional skinmechanismnemolizumabPrurigo nodularisregulatory mechanismsingle-cell ATAC sequencingsingle-cell RNA sequencing

Identifiers

PMID40939757
PMCPMC13261850

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.