Evidence map›Paper›PMID 40939596›Full record

ArticleStem cell reports2025

Trophoblast stem cell derivation from naive and primed hPSC enables ELF5 functional analysis.

Joonas Sokka, Ella Lapinsuo, Jouni Kvist, Sami Jalil, Masahito Yoshihara, Jere Weltner, Fredrik Lanner, Juha Kere, Diego Balboa, Timo Otonkoski and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Joonas SokkaResearch Programs Unit, Stem Cells and Metabolism and Biomedicum Stem Cell Centre, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland.
Ella LapinsuoResearch Programs Unit, Stem Cells and Metabolism and Biomedicum Stem Cell Centre, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland.
Jouni KvistResearch Programs Unit, Stem Cells and Metabolism and Biomedicum Stem Cell Centre, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland.
Sami JalilResearch Programs Unit, Stem Cells and Metabolism and Biomedicum Stem Cell Centre, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland.
Masahito YoshiharaDepartment of Medicine Huddinge, Karolinska Institutet, Huddinge, SE-14183 Stockholm, Sweden; Institute for Advanced Academic Research (IAAR), Chiba University, Chiba 260-8670, Japan; Department of Artificial Intelligence Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan; Premium Research Institute for Human Metaverse Medicine (WPI-PRIMe), The University of Osaka, Suita, Osaka 565-0871, Japan.
Jere WeltnerResearch Programs Unit, Stem Cells and Metabolism and Biomedicum Stem Cell Centre, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland; Folkhälsan Research Center, 00290 Helsinki, Finland.
Fredrik LannerDepartment of Clinical Science, Intervention and Technology, Karolinska Institutet, and Division of Obstetrics and Gynecology, Karolinska. Universitetssjukhuset, Stockholm, Sweden.
Juha KereResearch Programs Unit, Stem Cells and Metabolism and Biomedicum Stem Cell Centre, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland; Department of Medicine Huddinge, Karolinska Institutet, Huddinge, SE-14183 Stockholm, Sweden; Folkhälsan Research Center, 00290 Helsinki, Finland.
Diego BalboaResearch Programs Unit, Stem Cells and Metabolism and Biomedicum Stem Cell Centre, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland.
Timo OtonkoskiResearch Programs Unit, Stem Cells and Metabolism and Biomedicum Stem Cell Centre, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland; Children's Hospital, Helsinki University Central Hospital, University of Helsinki, 00290 Helsinki, Finland.
Ras TrokovicResearch Programs Unit, Stem Cells and Metabolism and Biomedicum Stem Cell Centre, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland. Electronic address: ras.trokovic@helsinki.fi.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human pluripotent stem cells (hPSCs) are valuable tools for studying placental biology, yet their differentiation into bona fide trophoblast stem cells (TSCs) remains challenging. In this study, we established and thoroughly compared naive and primed-derived TSC-like cells with primary human TSCs derived from pre-implantation blastocyst and first-trimester placenta. Comprehensive analyses confirmed expression of trophoblast lineage-specific genes and typical placental features. Detailed transcriptional analyses revealed that naive-derived TSC-like cells resembled embryo and placenta-derived cell lines and differentiated faster and more directly into TSC than primed-derived cells. We used these TSC-like models to study the role of ELF5, a transcription factor indispensable for maintenance and differentiation in mouse TSC. In contrast to the mouse, knockout and knockdown experiments revealed that ELF5 is dispensable for human TSC-like cells self-renewal and differentiation. Our study provides valuable transcriptional data and highlights the utility of hPSC-derived TSC-like cells for modeling the placenta and studying gene function.

Indexed as

DNA-Binding ProteinsPluripotent Stem CellsTranscription FactorsTrophoblastsAnimalsCell DifferentiationFemaleHumansMicePlacentaPregnancyDNA-Binding ProteinsELF5 protein, humanTranscription FactorsC19MCCRISPR-Cas9early embryon annotationELF5embryonic stem cellinduced pluripotent stem cellpluripotent stem cellsingle cell RNA sequencingtranscriptomicstrophoblasttrophoblast stem cell

Identifiers

PMID40939596
PMCPMC12790726

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.