ArticleNeuron2025
Absolute measurement of fast and slow neuronal signals with fluorescence lifetime photometry at high temporal resolution.
Article in Neuron, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Article
- Illuminating the Future of Catecholamine Detection.Journal of neurochemistry · 2026Review
- Hunger modulates exploration through suppression of dopamine signaling in the tail of the striatum.Neuron · 2025Article
- Timescales of dopamine release in the striatum as a window into hierarchical control.Current opinion in behavioral sciences · 2025Article
- Elevated synaptic PKA activity and abnormal striatal dopamine signaling in Akap11 mutant mice, a genetic model of schizophrenia and bipolar disorder.Nature communications · 2025Article
- Elevated synaptic PKA activity and abnormal striatal dopamine signaling inbioRxiv : the preprint server for biology · 2025Article
- Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.eLife · 2025Article
- Sensitive dLight3 for imaging broad-spectrum dopamine events across brain regions.Research square · 2025Article
- The promise and peril of comparing fluorescence lifetime in biology revealed by simulations.bioRxiv : the preprint server for biology · 2025Article
- Slow-Timescale Regulation of Dopamine Release and Mating Drive Over Days.bioRxiv : the preprint server for biology · 2025Article
- An integrated microfluidic and fluorescence platform for probing in vivo neuropharmacology.Neuron · 2025Article
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14 authors.
Funding
Abstract
Dynamic signaling by extracellular and intracellular molecules impacts downstream pathways in a cell-type-specific manner. Fluorescent reporters of such signals are typically optimized to detect fast, relative changes in concentration of target molecules. They are less well suited to detect slowly changing signals and rarely provide absolute measurements. Here, we developed fluorescence lifetime photometry at high temporal resolution (FLIPR), which utilizes frequency-domain analog processing to measure the absolute fluorescence lifetime of genetically encoded sensors at high speed but with long-term stability and picosecond precision. We applied FLIPR to investigate dopamine signaling in functionally distinct striatal subregions. We observed higher tonic dopamine levels in the tail of the striatum compared with the nucleus accumbens core and differential and dynamic responses in phasic and tonic dopamine to appetitive and aversive stimuli. Thus, FLIPR reports fast and slow timescale neuronal signaling in absolute units, revealing previously unappreciated spatial and temporal variation even in well-studied signaling systems.
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Registered trials
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