Evidence map›Paper›PMID 40938747›Full record

ArticleSTAR protocols2025

Protocol to record single-unit sensory neurons in an intact mouse ex vivo muscle-nerve-DRG-spinal cord preparation.

Adam J Dourson, Michael P Jankowski

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Adam J DoursonDepartment of Anesthesiology, Washington University School of Medicine, St. Louis, MO 63130, USA; Washington University Pain Center, St. Louis, MO 63130, USA; Department of Anesthesia, Division of Pain Management, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA.
Michael P JankowskiDepartment of Anesthesia, Division of Pain Management, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA; Pediatric Pain Research Center, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229, USA; Department of Pediatrics, University of Cincinnati, College of Medicine, Cincinnati, OH 45229, USA. Electronic address: michael.jankowski@cchmc.org.

Funding

Sensitization of developing sensory neurons after incisionR01NS105715 · NINDS · CINCINNATI CHILDRENS HOSP MED CTR · PI Michael P Jankowski · 2019 to 2026
$3.3M
Mechanisms of muscle afferent sensitization after ischemiaR01NS113965 · NINDS · CINCINNATI CHILDRENS HOSP MED CTR · PI JANKOWSKI, MICHAEL P · 2020 to 2024
$2.4M
NINDS NIH HHS R01 NS105715NINDS NIH HHS R01 NS113965
6 · The paper itself

Abstract

Dorsal root ganglia (DRG) neurons innervate peripheral tissues to relay tactile, thermal, and chemical information to the central nervous system (CNS). Here, we present a protocol to dissect an ex vivo preparation that retains anatomical innervation patterns to the hind paw muscles of the mouse. We also describe steps for directly recording single DRG neurons while applying physiologically relevant stimuli to their receptive fields. This protocol provides important information for studying sensory input from primary afferents. For complete details on the use and execution of this protocol, please refer to Dourson et al.

Indexed as

Ganglia, SpinalMuscle, SkeletalSensory Receptor CellsSpinal CordAnimalsMiceDevelopmental biologyNeuroscienceSystems biology

Identifiers

PMID40938747
PMCPMC12491161

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.