Evidence map›Paper›PMID 40938106›Full record

ArticleLab on a chip2025

Topographic cues regulate collective cell dynamics in curved nano/microgrooved tubular microchannels.

Tatsuya Matsubara, Chris P Miller, Chanhong Min, Chia-Yi Su, Jong Seob Choi, Chwee Teck Lim, Jude M Phillip, Joon-Wan Kim, Deok-Ho Kim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lab on a chip, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Tatsuya MatsubaraDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.ORCID 0000-0003-0408-8751
Chris P MillerTranslational Science and Therapeutics Division, Fred Hutchinson Cancer Center, Seattle, WA 98109, USA.
Chanhong MinDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Chia-Yi SuDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Jong Seob ChoiDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.ORCID 0000-0003-1621-1497
Chwee Teck LimDepartment of Biomedical Engineering, National University of Singapore, Singapore 117583, Singapore.ORCID 0000-0003-4019-9782
Jude M PhillipDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
Joon-Wan KimLaboratory for Future Interdisciplinary Research of Science and Technology (FIRST), Institute of Integrated Research (IIR), Institute of Science Tokyo, Yokohama, Kanagawa 226-8503, Japan.
Deok-Ho KimDepartment of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.ORCID 0000-0002-6989-6074

Funding

Engineered biomimetic collective cancer invasion models for screening chemotherapeutic agentsR01CA279948 · NCI · JOHNS HOPKINS UNIVERSITY · PI Eun Hyun Ahn, Deok-Ho Kim · 2024 to 2026
$2.1M
Defining a biophysical basis for cell types, cell states and cellular heterogeneity at single-cell resolutionR35GM157099 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Jude Marvin Phillip · 2025 to 2026
$762k
NCI NIH HHS R01 CA279948NIGMS NIH HHS R35 GM157099
6 · The paper itself

Abstract

Physical properties of the extracellular matrix, such as topography and curvature, regulate collective epithelial behaviors. However, the interplay between these geometric factors on collective migration is not well understood. In this study, we investigate the effects of topographic cues on a curved surface on collective epithelial migration within tubular microchannels with an inner diameter of 100 μm. These tubular microchannels feature circumferential or longitudinal micro- and nano-grooves fabricated by two-photon polymerization three-dimensional printing and micro-molding techniques. Live cell microscopy records the collective migration of GFP-labeled epithelial cells into the microchannel with each topographical design. We utilized a single-cell behavior analysis for the tracked time-dependent cell position data to visualize and quantify complex cell migration. Results show that longitudinal grooves (800 nm and 4 μm) enhanced cell migration, but circumferential grooves did not significantly enhance cell migration. This indicates that curvature rather than topography dominates migration at the microtube scale. These findings provide insights into the interplay between curvature, microscale structure, and cell behaviors and suggest the potential to control cell behaviors by manipulating the structure and topographic cues with their local microenvironments.

Indexed as

Lab-On-A-Chip DevicesAnimalsCell MovementEpithelial CellsMadin Darby Canine Kidney CellsSurface Properties

Identifiers

PMID40938106
PMCPMC12427609

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.