Evidence map›Paper›PMID 40937854›Full record

ArticlemBio2025

Naturally occurring mutations in envelope mediate virulence of Usutu virus.

Megan B Vogt, Jeffrey M Marano, William J Hanrahan, Seth A Hawks, Anne M Brown, Sheryl Coutermarsh-Ott, James Weger-Lucarelli, Nisha K Duggal

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Megan B VogtDepartment of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.ORCID 0000-0002-4520-3077
Jeffrey M MaranoDepartment of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.
William J HanrahanDepartment of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.
Seth A HawksDepartment of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.
Anne M BrownDepartment of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.ORCID 0000-0001-6951-8228
Sheryl Coutermarsh-OttDepartment of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.
James Weger-LucarelliDepartment of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.ORCID 0000-0002-9483-1694
Nisha K DuggalDepartment of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia, USA.ORCID 0000-0002-9893-8069

Funding

Evolution and pathogenesis of Usutu virus, an emerging arbovirusR21AI153988 · NIAID · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI DUGGAL, NISHA · 2021 to 2022
$423k
NIAID NIH HHS R21 AI153988
6 · The paper itself

Abstract

Usutu virus (USUV) is a mosquito-transmitted flavivirus that is closely related to West Nile virus. Recently, USUV emerged in Europe, where it has caused multiple bird die-off events and neuroinvasive disease in humans. Previously, we showed that USUV isolates from Africa cause significantly more severe disease than European isolates in mice. Sequence analysis revealed that the most virulent isolate (Uganda 2012) and the least virulent isolate (Netherlands 2016) differed by 21 amino acids across the viral polyprotein. Here, we sought to identify the viral determinants of and mechanisms for differential virulence. To accomplish this, we used our USUV reverse genetics system and bacteria-free cloning to generate chimeric viruses between Uganda 2012 and Netherlands 2016.

Indexed as

FlavivirusViral EnvelopeViral Envelope ProteinsVirulenceAnimalsFemaleFlavivirus InfectionsMaleMiceMutationPhylogenyViral Envelope Proteinschimeric virusesflavivirusUsutu virusviral determinantsvirulence

Identifiers

PMID40937854
PMCPMC12506040

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.