Evidence map›Paper›PMID 40937577›Full record

ArticleInternational journal of molecular medicine2025

CDC5L binds to ELAVL1 to inhibit pyroptosis in hepatocellular carcinoma through the Caspase 3/GSDME signaling pathway.

Shuai Liang, Zhongcheng Zhu, Yangshuo Tang, Shuhua Zhou, Moyan Xiao, Xuejun Gong, Ke Ye

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shuai LiangDepartment of Bile and Pancreatic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Zhongcheng ZhuDepartment of Bile and Pancreatic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Yangshuo TangDepartment of Ultrasound Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Shuhua ZhouComprehensive Surgery, Xiangya Boai Rehabilitation Hospital, Changsha, Hunan 410100, P.R. China.
Moyan XiaoDepartment of Hepatology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Xuejun GongDepartment of Bile and Pancreatic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Ke YeDepartment of Hepatology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hepatocellular carcinoma (HCC) is associated with elevated rates of illness and mortality, posing a significant challenge to global health. Elevated levels of cell division cycle 5 like (CDC5L) in HCC predict a poor prognosis. The present study aimed to elucidate the role of CDC5L in HCC. First, CDC5L expression was detected using bioinformatics analysis and validated on clinical samples and cells. Next, CDC5L was knocked down and overexpressed. CDC5L and Caspase 3 knockdown was conducted to explore the effect on pyroptosis in HCC. The mechanisms of HCC pyroptosis were investigated by interfering with and overexpressing ELAV like RNA binding protein 1 (ELAVL1), as well as interfering with CDC5L and overexpressing ELAVL1. Finally, the role of CDC5L/ELAVL1 in regulating Caspase 3/Caspase 3‑-gasdermin E (GSDME) in HCC pyroptosis was validated through cellular and animal experiments. It was found that CDC5L expression was increased in HCC. Elevated CDC5L levels were linked to adverse outcomes in HCC. Suppressing CDC5L expression reduced cell viability, curbed cell proliferation and diminished cell migration and invasion capabilities, while simultaneously enhancing pyroptosis. Additionally, CDC5L knockdown resulted in a progressive decline in tumor volume and a decrease in tumor size and weight. CDC5L overexpression exerted an opposite effect. Moreover, regulating pyroptosis in HCC by CDC5L was dependent on Caspase family. Following CDC5L knockdown, tumor volume steadily diminished, tumor mass shrank and tumor weight was correspondingly reduced. ELAVL1 overexpression reversed these effects. CDC5L competitively bound to ELAVL1 to inhibit the binding of ELAVL1 with

Indexed as

Carcinoma, HepatocellularCaspase 3Cell Cycle ProteinsELAV-Like Protein 1Liver NeoplasmsNeoplasm ProteinsPyroptosisSignal TransductionAnimalsCell Line, TumorCell ProliferationFemaleGasderminsGene Expression Regulation, NeoplasticHumansMaleCASP3 protein, humanCaspase 3CDC5L protein, humanCell Cycle ProteinsELAVL1 protein, humanELAV-Like Protein 1GasderminsGSDME protein, humanNeoplasm ProteinsRNA-Binding ProteinsCaspase 3Caspase 3‑gasdermin Ecell division cycle 5 likeELAV like RNA binding protein 1hepatocellular carcinomapyroptosis

Identifiers

PMID40937577
PMCPMC12459918

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.