ArticleSmall (Weinheim an der Bergstrasse, Germany)2025
Integrated Microfluidic Platform for Longitudinal Imaging, Functional Assays, and Downstream Proteomics in C. elegans.
Article in Small (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Article
- Integrated Microfluidic Platform for Longitudinal Imaging, Functional Assays, and Downstream Proteomics in C. elegans.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caenorhabditis elegans is a widely used model organism in developmental biology, neurobiology, and host-pathogen research. However, existing approaches for long-term, high-content imaging of individual worms are constrained by stress-inducing immobilization and fragmented observational workflows. Here, a microfluidic platform is introduced that enables continuous, high-resolution imaging of individual C. elegans from the L1 larval stage through adulthood within a fully integrated system. The device incorporates dynamic fluidic control for automated feeding, waste and progeny removal, and transient, non-invasive immobilization of up to 20 worms in parallel. On-chip nutrition is validated using fluorescently labeled bacteria, and physiological compatibility is confirmed through stress assays. Using sod-3p::GFP reporter strains, it is shown that short-term immobilization induces no detectable stress response, while starvation conditions produce strong activation. The platform also supports real-time pathogen tracking, demonstrated by visualizing the spatial distribution of the Pseudomonas aeruginosa PemB toxin in vivo. Finally, compatibility with single-nematode proteomics are established, identifying over 80 C. elegans proteins from individual worms despite bacterial background. Together, this system enables seamless integration of developmental monitoring, physiological assays, infection models, and molecular profiling at single-organism resolution. It offers a powerful and versatile tool for advancing microfluidics-enabled functional biology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.