Evidence map›Paper›PMID 40937394›Full record

ReviewIndian journal of clinical biochemistry : IJCB2025

A Review of Methionine and Cysteine: Their Roles as Sinks for Reactive Oxygen and Nitrogen Species, Including Bioinformatic Analysis of Their Percent Compositions in Metabolic Proteins.

Bethany F Laatsch, Burhan A Ali, Anna R Berthiaume, Grace C Cunningham, Sabrina M Duncan, Nathan Hau, Audrey Ho, Marais A Loomis, Harrison R Lowater, Brenya L McNally and 16 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Indian journal of clinical biochemistry : IJCB, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors.

Bethany F LaatschDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Burhan A AliDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Anna R BerthiaumeDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Grace C CunninghamDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Sabrina M DuncanDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Nathan HauDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Audrey HoDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Marais A LoomisDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Harrison R LowaterDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Brenya L McNallyDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Kate L MuellerDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Matt PoppitzDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Sandra A PrickettDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Sydney E SchroederDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Madison L SheplerDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Bryce H ShobergDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Carolyn G ShultDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Lucille S SmithDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Karissa L SnyderDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Joshua E SteczynskiDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Aka TortiDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Aidan VoonDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Moriah M WeissDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Trinity B WilsonDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Sudeep BhattacharyyaDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.
Sanchita HatiDepartment of Chemistry and Biochemistry, University of Wisconsin-Eau Claire, Eau Claire, USA.ORCID 0000-0002-7452-0872

Funding

Exploring the Dynamics of Prolyl-tRNA Synthetases: Towards Developing a Screening Method for Species-Specific InhibitorsR15GM117510 · NIGMS · UNIVERSITY OF WISCONSIN EAU CLAIRE · PI BHATTACHARYAY, SUDEEP, HATI, SANCHITA · 2016 to 2023
$878k
NIGMS NIH HHS R15 GM117510
6 · The paper itself

Abstract

This review focuses on the roles of the two primary sulfur-containing amino acids, cysteine and methionine, in regulating reactive oxygen/nitrogen species (RONS). RONS are highly reactive oxygen/nitrogen-containing free radicals and compounds. Endogenous and exogenous antioxidants, including sulfur-containing amino acids, protect cells against the harmful effects of RONS on cellular macromolecules. This study thoroughly reviews the mechanisms by which these two sulfur-containing amino acids neutralize RONS. Additionally, a bioinformatic analysis of the percentage compositions of cysteine and methionine in metabolic proteins of humans and 12 closely related species was conducted using a "Biopython" script to assess their potential role as sinks for RONS, maintaining the structure and function of metabolic proteins. A total of 119 proteins from various metabolic pathways, including glycolysis, pyruvate to acetyl CoA conversion, tricarboxylic acid cycle, oxidative phosphorylation, pentose phosphate pathway, gluconeogenesis, glycogen metabolism, fatty acid metabolism, amino acid catabolism, nucleotide biosynthesis, and ROS scavengers were included in the bioinformatics analysis. This review shows that methionine and cysteine play crucial roles in neutralizing RONS. The bioinformatic analysis revealed that the percentage compositions of methionine and cysteine are higher in key redox enzymes like dehydrogenases, enzymes involved in oxidative phosphorylation, and those participating in the committed steps of metabolic pathways. Supplementary Information: The online version contains supplementary material available at 10.1007/s12291-024-01284-y.

Indexed as

AntioxidantsOxidative stressReactive nitrogen speciesReactive oxygen speciesSulfur-containing amino acids

Identifiers

PMID40937394
PMCPMC12420569

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.