ArticleChina CDC weekly2025
Wastewater-Based Monitoring of Dengue Fever at Community Level - Guangzhou City, Guangdong Province, China, May 2024.
Article in China CDC weekly, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Methodological Approaches to Dengue Virus Detection in Wastewater: A Systematic Review and Meta-Analysis of Positivity Rate.Viruses · 2026Pooled it
- West Nile Virus: Epidemiology, Surveillance, and Prophylaxis with a Comparative Insight from Italy and Iran.Vaccines · 2026Review
- Clinical Characteristics of Hospitalized Patients with Dengue Fever in Guangzhou, China.Infection and drug resistance · 2026Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction: Traditional dengue surveillance operates reactively, frequently lagging behind viral transmission patterns and thereby impeding timely public health responses. Wastewater-based epidemiology (WBE) presents significant potential for proactive early warning systems. This study sought to implement and validate the first community-level WBE system for dengue during an active outbreak, evaluating its capacity to detect cryptic transmission and provide actionable intelligence for public health interventions. Methods: During a dengue virus serotype 1 (DENV-1) outbreak, we collected 618 wastewater grab samples from manholes within a 200-m radius of 8 reported cases, along with matched patient serum and urine samples. We systematically compared magnetic bead and polyethylene glycol (PEG) concentration methods for viral recovery efficiency. DENV-1 ribonucleic acid (RNA) was detected and quantified using reverse transcription quantitative polymerase chain reaction (RT-qPCR). Positive samples underwent genomic sequencing and phylogenetic analysis to confirm environmental signals and determine viral lineages. Results: The magnetic bead method demonstrated superior performance with a limit of detection of 10 copies/mL and was selected based on its higher recovery efficiency (59.7%). We successfully detected DENV-1 in 14 of 618 wastewater samples tested. Critically, a positive wastewater signal from one residential building preceded the clinical diagnosis of a new case within that same location by several hours. For a single patient, we successfully generated matched viral genomic sequences from serum, urine, and wastewater samples, providing definitive validation of the environmental signal's authenticity. Conclusions: Community-level wastewater surveillance represents a powerful and effective tool for dengue control programs. This approach provides actionable early warnings by detecting cryptic viral transmission before cases receive clinical identification. Such capabilities enable public health authorities to deploy preemptive, geographically-targeted interventions, including vector control measures, fundamentally improving both the speed and precision of outbreak responses while helping to mitigate disease spread.
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