ArticleBiochemistry and biophysics reports2025
In vitro evidence of HER2-selective cytotoxicity by scFv(Herceptin)-PE-STXA immunotoxin in gastric cancer cells.
Article in Biochemistry and biophysics reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- COL10A1 Overexpression Promotes Gastric Cancer Aggressiveness Through EMT and Major Oncogenic Pathways.International journal of molecular sciences · 2025Article
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5 authors.
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Abstract
The delivery of therapeutic agents in a targeted manner shows great potential for improving the degree of efficacy in cancer therapies by minimizing harm for normal tissues and reducing treatment-related complications. Among patients with gastric cancer, the HER2 receptor is expressed in approximately 10-30 % of cases. The scFv(Herceptin)-PE-STXA was cloned into the pET28a vector, followed by protein expression and purification. HEK-293 cells served as the control (HER2-negative), while NCI-N87 cells were utilized as the gastric cancer cells (HER2-positive). The cells were subjected to exposure different concentrations (35 and 50 μg/mL) of immunotoxin, after which growth was assessed using the MTT test. The capability of scFv(Herceptin)-PE-STXA to induce apoptotic pathway in NCI-N87 and HEK-293 cells was estimated through flow cytometry. Furthermore, the affinity for binding the IT, lactate dehydrogenase release, and the measurement of apoptosis pathway enzymes (caspase-9 and caspase-3 activities) were also conducted. The outcomes demonstrated that cytotoxic effects occurred in NCI-N87 cells with a dose-proportional manner approach, whereas no such impact was seen in HEK-293(P < 0.001). Flow cytometry analysis of NCI-N87 cells treated with scFv(Herceptin)-PE-STXA revealed a significant increase in apoptotic rate at dose of 35 and 50 μg/mL (55.6 % and 74.2 %). Additional analysis showed that exposing HER2-expressing NCI-N87 cells to the scFv(Herceptin)-PE-STXA caused a notable to enhance in apoptotic caspases activity (3 and 9) (P < 0.001). Results of our study indicated that the scFv(Herceptin)-PE-STXA induces apoptosis in the NCI-N87 cell line derived from gastric cancer. This study emphasizes the potential of scFv(Herceptin)-PE-STXA to specifically target HER2-expressing cancer cells.
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