Evidence map›Paper›PMID 40936501›Full record

ArticleGates open research2025

Mycobacterium tuberculosis growth inhibition by peripheral blood mononuclear cells from household contacts is not affected by previous SARS-CoV-2 infection.

Jane A Shaw, Caleb Petersen, Andriette Hiemstra, Maynard Meiring, Osagie A Eribo, Christian Otum, Ilana van Rensburg, Ayanda Shabangu, Bronwyn Smith, Firdows Noor and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Gates open research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jane A ShawStellenbosch University, Cape Town, South Africa.
Caleb PetersenStellenbosch University, Cape Town, South Africa.
Andriette HiemstraStellenbosch University, Cape Town, South Africa.
Maynard MeiringStellenbosch University, Cape Town, South Africa.
Osagie A EriboStellenbosch University, Cape Town, South Africa.
Christian OtumStellenbosch University, Cape Town, South Africa.
Ilana van RensburgStellenbosch University, Cape Town, South Africa.
Ayanda ShabanguStellenbosch University, Cape Town, South Africa.
Bronwyn SmithStellenbosch University, Cape Town, South Africa.
Firdows NoorStellenbosch University, Cape Town, South Africa.
Gerhard WalzlStellenbosch University, Cape Town, South Africa.
Kevin B UrdahlUniversity of Washington School of Medicine, Washington, Seattle, USA.
Dave LewinsohnOregon Health and Science University and Portland Veterans Administration Medical Center, Portland, Portland, USA.
Stephanus T MalherbeStellenbosch University, Cape Town, South Africa.
Nelita du PlessisStellenbosch University, Cape Town, South Africa.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: There is a concern that SARS-CoV-2 infection may drive poor outcomes after Methods: Twenty participants per group from Cape Town, South Africa were classified according to SARS-CoV-2 anti-S and anti-N antibody tests. We infected PBMC from each participant at a MOI of 0.001 with Mtb strain H37Rv in a 4-day growth inhibition assay. Mycobacteria were quantified through inoculation into Bactec Mycobacteria Growth Indicator Tube (MGIT) liquid culture. PBMC from a subset of participants were infected in the presence of autologous time-matched serum and Mtb-uninfected control PBMCs were included. Results: There was no difference in the time to detection of Mtb or the normalised Mtb growth ratio (log10CFUsample - log10CFUcontrol) between groups in the standard protocol, or when infected cells from the COV+HHC and COV+TB+ (n=10 each) groups were cultured with autologous time-matched serum. The group with active TB demonstrated the best Mtb growth control. Extracellular Mtb measured by culturing the supernatants of the infected cell cultures also did not show any difference between groups. Five (14.3%) uninfected controls were culture positive. Conclusion: Our results show that previous SARS-CoV-2 does not affect the Mtb killing ability of circulating mononuclear immune cells

Indexed as

COVID-19Leukocytes, MononuclearMycobacterium tuberculosisTuberculosisAdultFamily CharacteristicsFemaleHumansMaleMiddle AgedSARS-CoV-2South AfricacoinfectionCOVID-19immune responsekillingMtbperipheral blood mononuclear cellsSARS-CoV-2Tuberculosis

Identifiers

PMID40936501
PMCPMC12420909

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.