ArticleLab on a chip2025
Size-independent and automated single-colony-resolution microdroplet dispensing.
Article in Lab on a chip, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Microfluidics to Meet Antibiotic Resistance: A Growing Research Frontier.Antibiotics (Basel, Switzerland) · 2025Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Droplet microfluidics-based high-throughput screening (HTS) has proven to be an effective method for rapidly analyzing broad ranges of biological samples, such as cell libraries. However, the final step of the droplet microfluidics-based screening workflow, which is to dispense the sorted "hit" droplets to off-chip, ideally one "hit" droplet at a time, is prone to high error, especially when droplet size variation is present. Such size variations, often unavoidable in complex multi-step droplet microfluidics assay, lead to variations in droplet transition speed as they flow towards the dispensing tip, where smaller droplets move faster than larger droplets within a microchannel. Such difference in transit speed causes simultaneous dispensing of multiple droplets at a time instead of single-droplet dispensing, resulting in "hit" cells in a dispensed droplet being mixed with those from another "hit" droplet. To address this challenge, an approach that uses blank spacing droplets as physical barriers between "hit" droplets so that "hit" droplet-to-droplet distance remains the same throughout the droplet transition process is proposed here. These blank droplets (up to 1000) and a single "hit" droplet flow out of the droplet dispensing tip, forming a "drip", which is then dispensed one "drip" at a time into a well of a well plate or on an agar plate with sufficient drip-to-drip distance. This method of mixing empty droplets with "hit" droplets, combined with an off-the-shelf distance sensor that detects the formation of a "drip", which then moves the dispensing plate upward using a linear motor for the formed "drip" to be dispensed, enables precise and automated single-drip (
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.