Evidence map›Paper›PMID 40936345›Full record

ArticleMolecular microbiology2025

The Role of the [2Fe-2S] Cluster of Escherichia coli IscR in Responding to Redox-Cycling Agents.

Rajdeep Banerjee, Erin L Mettert, Angela S Fleischhacker, Patricia J Kiley

Abstract read
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In one paragraph

Article in Molecular microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Rajdeep BanerjeeDepartment of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Erin L MettertDepartment of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Angela S FleischhackerDepartment of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Patricia J KileyDepartment of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.ORCID 0000-0002-8771-1782

Funding

Bacterial anaerobic iron homeostasisR01GM115894 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI KILEY, PATRICIA J · 2015 to 2018
$1.2M
Studies of the regulation of IscR by [2Fe-2S] cluster bindingF32GM085987 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI FLEISCHHACKER, ANGELA SUE · 2008 to 2010
$143k
NIGMS NIH HHS F32 GM085987NIGMS NIH HHS R01 GM115894NIH HHS F32 GM085987NIH HHS R01 GM115894
6 · The paper itself

Abstract

The mechanisms by which cells respond to growth inhibitory redox-cycling agents is only partially understood. In Escherichia coli K12, the IscR regulon, which includes the ISC and SUF Fe-S cluster biogenesis machineries, is differentially expressed in response to these agents. Here, we report how one redox-cycling agent, phenazine methosulfate (PMS), regulates IscR activity via its [2Fe-2S] cluster cofactor. A direct role for IscR in mediating the response to PMS was inferred from the PMS-dependent weakening of [2Fe-2S]-IscR binding to an isc operon type 1 DNA site in vitro. This decrease in DNA binding was attributed to the accompanying oxidation of its [2Fe-2S]

Indexed as

Escherichia coliEscherichia coli K12Escherichia coli ProteinsIron-Sulfur ProteinsTranscription FactorsAnaerobiosisGene Expression Regulation, BacterialOperonOxidation-ReductionPromoter Regions, GeneticRegulonEscherichia coli ProteinsIron-Sulfur ProteinsIscR protein, E coliTranscription FactorsFe‐S cluster biogenesisFe‐S cluster homeostasisredox regulationRrf2 family of transcription factors

Identifiers

PMID40936345
PMCPMC12594620

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.