Mycobacterial Tyrosine Phosphatase PtpB Affects Host Cytokine Expression by Dephosphorylating ERK1/2 and STAT3.

Tianxian Liu, Yameng Fan, Yijia Chen, Shuyu Xie, Jun-Yu Xu, Minjia Tan, Bang-Ce Ye

Abstract read

In one paragraph

Article in Molecular & cellular proteomics : MCP, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

The functions and inhibitors of protein tyrosine phosphatase B fromFrontiers in immunology · 2026
Review
Secreted proteins ofFrontiers in tuberculosis · 2026
Review

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Tianxian LiuLaboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China; State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Yameng FanState Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Yijia ChenLaboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China.

Shuyu XieState Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.

Jun-Yu XuState Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China; Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan, China. Electronic address: jyxu@simm.ac.cn.

Minjia TanState Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China; Zhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Zhongshan, China. Electronic address: mjtan@simm.ac.cn.

Bang-Ce YeLaboratory of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China. Electronic address: bcye@ecust.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycobacterium tuberculosis (Mtb) tyrosine phosphatases PtpA and PtpB have been widely reported to affect host immunity response and bacterial intercellular survival. However, a comprehensive investigation into the impact of PtpA and PtpB on host phosphorylation, specifically in their roles as tyrosine phosphatases, has not yet been reported. In this study, we first conducted the potential dephosphorylation substrates map of PtpA and PtpB within the host. Our findings demonstrated that PtpB significantly decreased the phosphorylation levels of ERK1/2 and STAT3. Subsequent analysis indicated that PtpB modulated the production of cytokine TNF and IL-1β by dephosphorylating ERK1/2 and preventing its nuclear translocation. PtpB also reduced IL-6 and IL-1β expression by dephosphorylating STAT3. The in vivo experiment demonstrated increased bacterial survival and reduced cytokine expression in the PtpB-overexpression strain. Consequently, our findings demonstrate that Mtb tyrosine phosphatases PtpA and PtpB play critical roles in the global tyrosine phosphorylation landscape within the host. Specifically, PtpB modulates cytokine expression through the dephosphorylation of ERK1/2 and STAT3.

Indexed as

Bacterial ProteinsCytokinesMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mycobacterium tuberculosisProtein Tyrosine PhosphatasesSTAT3 Transcription FactorAnimalsHost-Pathogen InteractionsHumansInterleukin-1betaMicePhosphorylationBacterial ProteinsCytokinesInterleukin-1betaMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MptpA protein, Mycobacterium tuberculosisProtein Tyrosine PhosphatasesSTAT3 Transcription FactorERK1/2immune responsemycobacteriatyrosine phosphatases PtpBtyrosine phosphoproteomic

Identifiers

PMID40935154

DOI10.1016/j.mcpro.2025.101067

PMCPMC12546780

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