Prevalence and risk factors of intestinal protozoal infections among patients in Malaysia: A systematic review and meta-analysis.

backgroundsIntestinal protozoan infections (IPI), including Entamoeba spp., Giardia lamblia and Cryptosporidium spp., are common in diarrhoea patients in Malaysia. These parasites are primarily transmitted through contaminated food and water sources and pose a significant public health concern. Entamoeba spp., causes amoebiasis, which is characterised by severe diarrhoea with bloody stools, while Giardia spp., causes giardiasis, which is characterised by watery diarrhoea, abdominal pain and flatulence. Cryptosporidium spp. causes cryptosporidiosis, which is particularly severe in immunocompromised individuals. Despite efforts to improve water quality, sanitation, hygiene, and surveillance, limited epidemiological data prevent a clear understanding of the prevalence of intestinal protozoa and response to treatment. This systematic review and meta-analysis aimed to estimate prevalence, identify risk factors, and evaluate detection methods.

methodsA comprehensive literature search was carried out in the following databases: Scopus, Google Scholar, Web of Science, PubMed and Cochrane Library. The review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidance. Based on the random effects model, results were reported as proportions with 95% confidence intervals (CI). Subgroup analyses were performed based on detection methods, target populations and regions.

resultsA total of 103 articles were found on the prevalence and risk factors of IPI in Malaysia. After removing duplicates and screening for eligibility, 49 studies were included in this meta-analysis. The overall pooled prevalence of IPI in Malaysia was 24% (95% CI: 0.17.0, 0.29). with I2 = 98.94%, (P < 0.001). Among the identified protozoa, Entamoeba spp. had the highest prevalence at 18% (95% CI: 0.12, 0.24), followed by G. lamblia at 11% (95% CI: 0.08, 0.14) and Cryptosporidium spp. at 9% (95% CI: 0.03, 0.14). A subgroup analysis revealed that Kelantan and Perak state have the highest prevalence of 39% and 29% while Selangor and Kuala Lumpur reported the lowest (13.6%). The highest prevalence was observed in the indigenous communities (27%), followed by the local communities that mainly comes from rural area (23%). According to a meta-analysis of ten risk factors, the pooled prevalence of protozoal intestinal infections was significantly higher (between 38% and 52%) in children under 15 years of age, in males, in those with low income or no formal education, and in those exposed to untreated water, poor sanitation or unhygienic practises. A high level of heterogeneity was observed (I2 > 98%), reflecting substantial variability across the included studies.

conclusionThis review provides valuable insights into the epidemiology of protozoal intestinal infections in Malaysia. The high pooled prevalence of 24% underscores a substantial and ongoing burden of intestinal protozoal infections in Malaysia. The pooled prevalence should be interpreted with caution due to high heterogeneity, as the findings may not be generalizable to all settings. These findings support the development of evidence-based interventions to reduce the impact of these infections. Targeted screening, improved diagnostics, better access to clean water and sanitation, and health education for vulnerable groups are essential to reduce the burden of intestinal protozoa and strengthen national control measures.