Evidence map›Paper›PMID 40934011›Full record

ArticleFrontiers in cellular and infection microbiology2025

Construction of an EGFP-embedded porcine reproductive and respiratory syndrome virus infectious clone and antiviral drug screening.

Rongxiao Liu, Shi Fu, Yukun Chai, Nian Liu, Benjin Liu, Lingzhi Luo, Wei Yang, Xiumei Dong, Jin Cui

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Rongxiao LiuCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Shi FuCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Yukun ChaiCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Nian LiuCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Benjin LiuCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Lingzhi LuoCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Wei YangCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Xiumei DongCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.
Jin CuiCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant pathogen posed a serious threat to the global swine industry. In this study, a BAC-based reverse genetics platform was established using a highly pathogenic PRRSV (HP-PRRSV) strain. Three recombinant reporter viruses were constructed by inserting the enhanced green fluorescent protein (EGFP) gene into three different intergenic regions of the complete PRRSV-L251 genome. Immunofluorescence assays combined with viral growth kinetics and reporter gene stability assessments indicated that rL251-ORF4-5a-EGFP maintained relatively stable expression during serial passage, and viral titers at 72 hours post-infection (hpi) were comparable to the parental virus. Subsequently, we identified four candidate compounds with potential anti-PRRSV activity using rPRRSV-L251-ORF4-5a-EGFP, indicating that this platform can be used as a visual assessment tool for antiviral drug screening. This study demonstrated that the ORF4-5a interval region is a feasible and promising site for exogenous gene insertion, and provided a robust technical platform for PRRSV vaccine development and pathogenesis studies.

Indexed as

Antiviral AgentsGreen Fluorescent ProteinsPorcine respiratory and reproductive syndrome virusAnimalsCell LineDrug Evaluation, PreclinicalGenes, ReporterPorcine Reproductive and Respiratory SyndromeReverse GeneticsSwineVirus ReplicationAntiviral Agentsenhanced green fluorescent proteinGreen Fluorescent Proteinsantiviral drug screeningBACEGFPPRRSVreverse genetics system

Identifiers

PMID40934011
PMCPMC12417477

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.