Evidence map›Paper›PMID 40932770›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2025

Directed evolution of a plant Rubisco chaperone with altered client recognition.

Siyu Li, ByungUk Lee, Yichong Lao, Sirawit Lertwiriyapiti, Xuhui Huang, Tina Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Siyu LiDepartment of Biochemistry, University of Wisconsin, Madison, WI 53706.ORCID 0009-0007-1674-1419
ByungUk LeeDepartment of Chemistry, University of Wisconsin, Madison, WI 53706.
Yichong LaoBiophysics Graduate Program, University of Wisconsin, Madison, WI 53706.ORCID 0000-0002-1895-1467
Sirawit LertwiriyapitiDepartment of Chemistry, University of Wisconsin, Madison, WI 53706.
Xuhui HuangDepartment of Chemistry, University of Wisconsin, Madison, WI 53706.ORCID 0000-0002-7119-9358
Tina WangDepartment of Biochemistry, University of Wisconsin, Madison, WI 53706.ORCID 0000-0001-7259-7449

Funding

National Aeronautics and Space Administration (NASA) 80NSSC21K0065Wisconsin Alumni Research Foundation (WARF) N/A
6 · The paper itself

Abstract

Improving the Calvin-Benson-Bassham cycle enzyme Ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) has the potential to increase crop productivity. However, the selectivity of the chaperones mediating Rubisco assembly in vascular plants toward their cognate Rubisco presents a substantive roadblock to both Rubisco protein engineering and transgenic expression of heterologous Rubisco orthologs, both of which necessitate changes to the Rubisco sequence. Here, we ask whether a plant Rubisco chaperone can be reprogrammed by directed evolution to accommodate a nonnative client. We developed a selection strategy to assess Rubisco assembly factor activity in high-throughput and used this selection to identify mutants of the chaperone Raf1 from

Indexed as

ArabidopsisArabidopsis ProteinsDirected Molecular EvolutionMolecular ChaperonesNicotianaRibulose-Bisphosphate CarboxylasePhotosynthesisArabidopsis ProteinsMolecular ChaperonesRibulose-Bisphosphate Carboxylasechaperonesdirected evolutionprotein engineeringRubisco

Identifiers

PMID40932770
PMCPMC12452902

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.