Evidence map›Paper›PMID 40932491›Full record

ArticleApplied microbiology and biotechnology2025

Foot-and-mouth disease virus vaccine with VP1 G-H loop substitution of the Cathay strain broadens antigen spectrum.

Jingjing Zha, Keqiang Zhang, Xingze Zhang, Pu Sun, Dong Li, Yimei Cao, Xingwen Bai, Xueqing Ma, Kun Li, Hong Yuan and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Jingjing ZhaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Keqiang ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Xingze ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Pu SunState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Dong LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Yimei CaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Xingwen BaiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Xueqing MaState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Kun LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Hong YuanState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Yuanfang FuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Jing ZhangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Zhixun ZhaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Jian WangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Huifang BaoState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Yifan OuYangState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Shuyun QiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Zaixin LiuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China.
Zengjun LuState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China. luzengjun@caas.cn.
Pinghua LiState Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730000, Gansu Province, China. lipinghua@caas.cn.

Funding

This work was financially supported by the National Program on Key Research Project of China. (2023YFD1802501)
6 · The paper itself

Abstract

Recently, serotype O foot-and-mouth disease viruses (FMDVs) belonging to four lineages (O/ME-SA/PanAsia, O/ME-SA/Ind2001, O/SEA/Mya-98, and O/Cathay) co-circulate in China, and the emergence of new variant viruses belonging to the Cathay lineage renders the existing vaccines less effective, which poses a serious threat to the livestock industries. The surface-exposed G-H loop of the VP1 structural protein of FMDV plays an important role in inducing neutralizing antibodies, generation of antigenic variants, and virus attachment. Here, we generated three recombinant FMDVs in which the G-H loops have been substituted with the corresponding sequences from the circulating strains of the PanAsia, Mya-98, and Cathay lineages based on an infectious cDNA clone of a chimeric FMDV, which carries amino acid substitutions in the leader protein and all surface proteins of FMDV O/XJ/CHA/2017. All mutant viruses exhibited a significantly improved replication capacity in BHK-21 cells and displayed relatively larger plaque morphologies compared with the parental virus. Chemically inactivated vaccines prepared from the parental virus and the mutant viruses all induced protective liquid-phase blocking ELISA (LPBE) antibodies against FMDVs in pigs after 28 days post vaccination (dpv), but only the vaccine with the substitution of the G-H loop of the Cathay strain induced protective neutralizing antibodies against the viruses of four lineages, while other vaccines exhibited excellent immunological cross-reactivity to the viruses of Ind2001, PanAsia, and Mya-98 lineages but did not for the Cathay virus at 28 dpv. Our studies indicated that the G-H loop of the Cathay virus plays a critical role in the antigenic drift of FMDV, which will provide key insights for designing porcinophilic FMDV vaccines in the future. KEY POINTS: • The swapping of the G-H loops of FMDVs notably improved replication capacity of FMDV in BHK-21 cells. • The swapping of the G-H loop of the Cathay virus obviously broadens antigenic coverage of FMDV vaccine. • The study showed that the G-H loop of the Cathay virus plays a critical role in antigenic drift of FMDV.

Indexed as

Antigens, ViralCapsid ProteinsFoot-and-Mouth DiseaseFoot-and-Mouth Disease VirusViral VaccinesAmino Acid SubstitutionAnimalsAntibodies, NeutralizingAntibodies, ViralAntigenic VariationCell LineChinaCricetinaeSwineVaccines, InactivatedAntibodies, NeutralizingAntibodies, ViralAntigens, ViralCapsid ProteinsVaccines, InactivatedViral VaccinesVP1 protein, Foot-and-mouth disease virusBroaden antigenic coverageFoot-and-mouth disease virusG-H loopO/CathaySubstitution

Identifiers

PMID40932491
PMCPMC12426072

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.