Evidence map›Paper›PMID 40932289›Full record

ArticleMicrobiology spectrum2025

Hierarchical integration of mNGS, PCR, and other conventional methods for precision TB diagnostics.

Yating Zhao, Longting Du, Junli Song, Wei Sun, Yili Chen, Xuegao Yu, Hao Huang, Gang Huang, Enpu Huang, Ni Wang and 3 more

Abstract read
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Observational
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yating Zhao *Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.ORCID 0009-0008-3512-0412
Longting Du *Department of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
Junli Song *Reproductive Medicine Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
Wei SunDepartment of Laboratory Medicine, Maternal and Child Health Hospital of Qingyuan City, Qingyuan, Guangdong, China.
Yili ChenDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
Xuegao YuDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
Hao HuangDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.
Gang HuangOrgan Transplant Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.ORCID 0000-0002-7686-8434
Enpu HuangDepartment of Laboratory Medicine, The First People's Hospital of Fangchenggang City, Fangchenggang, Guangxi, China.
Ni WangDepartment of Laboratory Medicine, The Second People's Hospital of Qujing City, Qujing, Yunnan, China.
Shu AnDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.ORCID 0009-0005-8444-0576
Lu AiDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.ORCID 0009-0004-3816-637X
Peisong ChenDepartment of Laboratory Medicine, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong, China.ORCID 0000-0003-0640-3663

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2023A1515012526Basic and Applied Basic Research Foundation of Guangdong Province 2024A1515012332Clinical high-tech, major and special technology projects of Guangzhou 2023P-TS46Guangdong Provincial Center for Disease Control and Prevention Supports Talent Projects 0720240122
6 · The paper itself

Abstract

This study systematically compared the diagnostic accuracy of seven assays for detecting the IMPORTANCE: This study is the first to comprehensively evaluate the diagnostic efficacy, cost-effectiveness, and timeliness of seven TB detection methods in a single-center cohort. Our findings provide actionable solutions for optimizing TB diagnostics in diverse healthcare ecosystems, aligning with the WHO's End TB Strategy to ensure equitable access to rapid diagnostics.

Indexed as

High-Throughput Nucleotide SequencingMolecular Diagnostic TechniquesMycobacterium tuberculosisPolymerase Chain ReactionTuberculosisAdultAgedFemaleHumansMaleMetagenomicsMiddle AgedReal-Time Polymerase Chain ReactionRetrospective StudiesROC CurveSensitivity and SpecificityddPCREasyNATmNGSMTBCRT-qPCR

Identifiers

PMID40932289
PMCPMC12502707

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.