Evidence map›Paper›PMID 40931271›Full record

ArticleMolecular biotechnology2026

Expression, Purification and Biochemical Characterisation of Babesia bigemina Lactate Dehydrogenase.

Safiye Merve Bostancioglu, Ozkan Danis, Sinem Kocer, Ozal Mutlu

Abstract read
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In one paragraph

Article in Molecular biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Safiye Merve BostanciogluDepartment of Biology, Faculty of Science, Marmara University, Göztepe, 34722, Istanbul, Türkiye.
Ozkan DanisDepartment of Chemistry, Faculty of Science, Marmara University, Göztepe, 34722, Istanbul, Türkiye.
Sinem KocerDepartment of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul Yeni Yuzyil University, 34010, Istanbul, Türkiye.
Ozal MutluDepartment of Biology, Faculty of Science, Marmara University, Göztepe, 34722, Istanbul, Türkiye. ozal.mutlu@marmara.edu.tr.ORCID http://orcid.org/0000-0003-4551-5780

Funding

Marmara Üniversitesi FDK-2022-10061
6 · The paper itself

Abstract

Babesia bigemina, a tick-borne protozoan parasite, is one of the main causative agents of bovine babesiosis, a disease with significant economic impact on the cattle industry. One of the key enzymes involved in the parasite's metabolism is lactate dehydrogenase (LDH), which plays an essential role in the anaerobic glycolytic pathway by catalysing the conversion of pyruvate to lactate. In this study, B. bigemina LDH gene was cloned, expressed in Escherichia coli and subsequently purified using affinity chromatography. The purified enzyme was subjected to biochemical assays to determine its stability, optimal pH, thermostability, and kinetic parameters. Kinetic analyses indicated a Kₘ value of 0.2585 mM for pyruvate and a K

Indexed as

BabesiaL-Lactate DehydrogenaseProtozoan ProteinsAnimalsCattleCloning, MolecularEnzyme StabilityEscherichia coliGene ExpressionHydrogen-Ion ConcentrationKineticsNADRecombinant ProteinsTemperatureL-Lactate DehydrogenaseNADProtozoan ProteinsRecombinant ProteinsBabesia bigeminaBiochemical characterisationEnzyme kineticsLactate dehydrogenaseTick-borne protozoan

Identifiers

PMID40931271

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.