ArticleNucleic acids research2025
Visualizing intracellular glycine with two-dye and single-dye ratiometric RNA-based sensors.
Article in Nucleic acids research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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1 citing paper in PubMed.
- Genetically encoded tools for tracking metabolites in live cells.Biochemical Society transactions · 2026Review
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2 authors.
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Abstract
Glycine is an important metabolite and cell signal in diverse organisms, yet tools to visualize intracellular glycine dynamics have not been developed. In this study, diverse and bright RNA-based glycine biosensors were developed by fusing the architecturally complex glycine riboswitch with Broccoli class fluorogenic aptamers. The brightest sensor with the highest activation, glyS, and its two-dye ratiometric counterpart, Pepper-glyS, allowed for visualization of a drug-induced accumulation of endogenous glycine in live Escherichia colicells. However, a general limitation of two-dye orthogonal aptamer pairs is that differences in dye properties may prevent accurate quantitation of cellular targets. Here, a novel Golden Broccoli aptamer was developed that is readily resolved from Red Broccoli by spectral unmixing methods, even though both aptamers bind the same dye, DFHO. This enabled generation of the first single-dye ratiometric sensor, which detects glycine both in vitro and in live E. coli cells.
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