ArticleFrontiers in veterinary science2025
Analytical validation of a flow cytometric method for the detection and quantification of canine mast cells in peripheral blood, bone marrow, and lymph node.
Article in Frontiers in veterinary science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Mast cell tumors (MCTs) are the most common skin neoplasms in dogs and exhibit highly variable biological behavior. Metastasis primarily affects the lymph nodes, though less frequently, MCTs can infiltrate the spleen, liver, peripheral blood, and bone marrow. Flow cytometry of fine needle aspirate samples represents a non-invasive diagnostic procedure that has shown promise for detecting and quantifying mast cells in primary tumors and lymph nodes. However, analytical validation of this method for clinical use is lacking. This study aimed to evaluate the analytical performance of a flow cytometric panel for quantifying mast cells in peripheral blood, bone marrow, and lymph node aspirates from dogs. Key parameters as the limit of blank (LOB), lower limit of detection (LLoD), lower limit of quantification (LLoQ), intra-assay precision, and accuracy, were evaluated. The method demonstrated high precision across a wide range of mast cell concentrations, with analytical coefficient of variation (CV
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