ArticleApplied biosafety : journal of the American Biological Safety Association2025
Low-Energy Electron Irradiation Inactivates Viruses in Serum and Maintains Growth-Promoting Properties in Cell Culture.
Article in Applied biosafety : journal of the American Biological Safety Association, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Serum and other blood-derived products are widely used in biomedical and biopharmaceutical processes, especially for the production of vaccines or cell therapeutic applications. To ensure quality and safety, each serum lot undergoes testing for sterility to minimize the risk of disease transmission. A currently performed standard procedure is gamma-irradiation of serum for effectively killing pathogens. However, gamma irradiation can only be performed in highly shielded, specialized facilities. Low-energy electron irradiation (LEEI) is an alternative, Good Manufacturing Practice (GMP) compatible method for sterilizing serum in a standard laboratory environment. Results: We directly compared the sensitivity of viruses belonging to the family of flaviviruses (Zika virus and bovine viral diarrhea virus) and parvoviruses (minute virus of mice) against LEEI in 90% serum or buffer. Although the reduction is more effective in buffer than in serum, the calculated decimal reduction dose values suggest that LEEI can efficiently sterilize serum by inactivating viruses, thereby increasing product safety and extending shelf life. Furthermore, LEEI-treated serum was tested as a supplement in cell culture and showed comparable performance to nonirradiated or gamma-irradiated serum when used for the cultivation of different cell lines. Conclusion: LEEI is able to inactivate viral contaminants in serum and offers significant advantages over gamma irradiation, as no large amounts of radioactivity are generated, on-site irradiation is possible, and space requirements are reduced due to fewer protective measures. These advantages allow the technology to be used in a GMP-compliant irradiation process.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.