Evidence map›Paper›PMID 40926636›Full record

ArticleFEBS open bio2026

Establishment and optimization of the two-step induction system for generating primordial germ cell-like cells from chicken embryonic stem cells.

Zeyu Li, XianShuai Xu, GuangZheng Liu, XiaoQian Lv, JiuZhou Song, HongYan Sun, YingJie Niu, QiSheng Zuo, Wei Han, BiChun Li and 1 more

Abstract read
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Article in FEBS open bio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Zeyu LiJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, China.ORCID https://orcid.org/0009-0006-0209-6300
XianShuai XuJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, China.
GuangZheng LiuJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, China.
XiaoQian LvJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, China.
JiuZhou SongDepartment of Animal & Avian Sciences, University of Maryland, College Park, MD, USA.
HongYan SunJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, China.
YingJie NiuJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, China.
QiSheng ZuoJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, China.
Wei HanPoultry Institute, Jiangsu Institute of Poultry Sciences/Chinese Academy of Agricultural Sciences, Yangzhou, China.
BiChun LiJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, China.
Kai JinJoint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, China.ORCID https://orcid.org/0000-0003-2489-2173

Funding

STI 2030-Major Projects 2023ZD0407503The China Postdoctoral Science Foundation 2022M722697The "JBGS" Project of Seed Industry Revitalization in Jiangsu Province JBGS〔2021〕029The National Natural Science Foundation of China 32172718The National Natural Science Foundation of China 32202655
6 · The paper itself

Abstract

Primordial germ cells (PGCs) are the progenitor cells of sperm and eggs. Xenotransplantation of chicken PGCs can achieve germline transmission. However, there are still challenges in obtaining many PGCs from endangered birds in vitro. In this study, at first, by incorporating 2i factors, the embryonic stem cells (ESCs) culture conditions were optimized, successfully yielding and validating pluripotent ESCs clones. Then, during induction ESCs, bFGF, activin A, and 1% KSR were added to Epiblast-like cells (EpiLCs). Quantitative real-time polymerase chain reaction (qRT-PCR) showed Pax6, Eomes, and Vimentin expression patterns similar to primary epiblast, indicating successful EpiLCs induction. During EpiLCs to Primordial germ cell-like cells (PGCLCs) transformation, we evaluated BMP4, BMP8b, EGF, LIF, and SCF combinations' impact on induction efficiency. Flow cytometry, qRT-PCR, and immunofluorescence showed high expression of Cvh, C-kit, Dazl, CVH, and DAZL in PGCLCs, suggesting successful EpiLCs differentiation. Induced PGCLCs injected into 2.5-day chick embryos migrated to gonads by day 7-7.5, demonstrating migration and colonization. This study optimized a two-step protocol for in vitro differentiation of chicken ESCs into PGCLCs. This research's results not only provide a reference for obtaining many PGCLCs in vitro but also open up a new approach for the development and application of genetic resource preservation technology in domestic chickens.

Indexed as

Cell Culture TechniquesEmbryonic Stem CellsGerm CellsAnimalsCell DifferentiationChick EmbryoChickenschickenembryonic stem cellsoptimizationprimordial germ cell‐like cellstwo‐step induction system

Identifiers

PMID40926636
PMCPMC12767776

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