Evidence map›Paper›PMID 40926333›Full record

ArticleMicrobial biotechnology2025

A BtpB-Deleted Brucella Vaccine Strain Provides Dual Advantages: Enhanced Protection and Diagnostic Differentiation Capability in Mice.

Zhenyu Xu, Xiaoyu Deng, Hengyun Gao, Yueli Wang, Huan Zhang, Yimei Xu, Haibo He, Aodi Wu, Yuchen Zhang, Jihai Yi and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Zhenyu XuCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Xiaoyu DengHuaihua Key Laboratory of Ion Channels and Complex Diseases, School of Basic Medicine, Hunan University of Medicine, Huaihua, Hunan, China.
Hengyun GaoCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Yueli WangCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.ORCID https://orcid.org/0000-0001-5320-4953
Huan ZhangCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Yimei XuCenter for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China.
Haibo HeCenter for Disease Control and Prevention of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, China.
Aodi WuCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Yuchen ZhangCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Jihai YiCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Zhen WangCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Chuangfu ChenCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
Zhongchen MaCollege of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.

Funding

High-level Talent Research Project of Shihezi University RCZK202456Major Scientific and Technological Projects of Corps 2017AA003National Natural Science Foundation of China U1803236Talent Program 'Tianchi Talent (Young Doctor)' in Xinjiang Uygur Autonomous Region (CZ004336) CZ004336
6 · The paper itself

Abstract

The Brucella abortus A19 attenuated live vaccine poses potential infection risks during practical applications and interferes with serological diagnostics, thereby affecting quarantine measures and the establishment of disease-free zones. Consequently, this study aimed to reduce its potential virulence, enhance its protective efficacy and differentiate it from wild-type strains by knocking out the immunosuppressive virulence gene btpB in the A19 strain. Using homologous recombination, we successfully obtained the A19ΔBtpB deletion strain. In a mouse model, the A19ΔBtpB strain demonstrated improved safety and significantly increased TLR2 and TLR4 expression levels in splenic lymphocytes, suggesting attenuated immune suppression. The A19ΔBtpB strain induced Brucella-specific IgG antibodies comparable to those of the A19 strain but exhibited superior performance in promoting cellular immunity. It effectively induced Th1-type cytokine (IL-6 and TNF-α) production, reduced Th2-type cytokine (IL-4 and IL-10) expression and promoted IFN-γ expression in T lymphocytes. Notably, the A19ΔBtpB deletion strain provided better protection against the virulent M28 strain in mice than did the A19 strain. In addition, an indirect ELISA diagnostic method based on BtpB protein has been developed, effectively distinguishing vaccine and wild-type strains in the infection of Brucella. The A19ΔBtpB strain thus represents a promising candidate for a Brucella gene-deletion vaccine, offering both theoretical and empirical support for future vaccine development.

Indexed as

Bacterial ProteinsBrucella abortusBrucella VaccineBrucellosisVirulence FactorsAnimalsAntibodies, BacterialCytokinesDisease Models, AnimalFemaleGene DeletionImmunoglobulin GMiceMice, Inbred BALB CVaccines, AttenuatedAntibodies, BacterialBacterial ProteinsBrucella VaccineCytokinesImmunoglobulin GVaccines, AttenuatedVirulence FactorsBrucella abortus A19 strainBtpBiELISAimmunoprotection

Identifiers

PMID40926333
PMCPMC12420658

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.