ArticlePloS one2025
Evaluating antibody mediated opsonophagocytosis of bacteria via lab protocol: RAW 264.7 cell phagocytosis assay.
Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- Article
- Associations Between Systemic Immunity Inflammation Index, Aggregate Index of Systemic Inflammation, and Hospital Acquired Otitis Media in Children Aged ≤12 years with Bacterial Pneumonia.Infection and drug resistance · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Assessing the phagocytosis of microbes by macrophages is an important component of studies of novel immunotherapeutics, antimicrobial drugs, immune effectors, or any immunology related research. Here we define two protocols for measuring in vitro phagocytosis by RAW 246.7 cells - a photographic phagocytosis assay that allows optical measurement of bacterial cells inside of the RAW 246.7 cell by staining fixed cells and visually quantifying the bacteria that have been phagocytosed, as well as a killing assay, which measures the viable bacteria released from lysed cells following exposure and phagocytosis via colony forming unit analysis. These methods differ from previously available protocols for measuring phagocytosis, as they are more generalizable to a variety of microbes and experimental designs and use readily available cell lines and materials to produce robust results. We demonstrate the utility of our methods by showing how opsonization by our novel, therapeutic monoclonal antibodies drive phagocytosis and killing of the gram-negative bacteria, Acinetobacter baumannii.
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Registered trials
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