ArticleSTAR protocols2025
Protocol for imaging and quantifying single-cell CDK activity levels during early mouse embryonic development by time-lapse microscopy.
Article in STAR protocols, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- From Static to Dynamic: Fluorescence Imaging Technology Advances Precise Embryo Evaluation.Small methods · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tracking the translocation of fluorescent-based reporters at the single-cell level in living mouse embryos requires specialized expertise in mouse embryology and deep computational skills. Here, we detail an approach to quantify cyclin-dependent kinase (CDK) activity levels in single cells throughout different stages of the pre-implantation embryo. We discuss in vitro culture strategies that enable efficient live fluorescent confocal image acquisition and subsequent cell tracking. Finally, we describe the image analysis workflow needed for the processing and visualization of the datasets obtained. For complete details on the use and execution of this protocol, please refer to Saykali et al.
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Registered trials
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